ArticleCell2018
Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry.
Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
116 citing papers in PubMed.
- Cross-domain signal grammars of transcription termination.Advanced biotechnology · 2026Review
- Protein-Ratio Rheostats: Integrating Stoichiometry, Abundance, and Binding Affinity in Cellular Function.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Long-range mRNA folding shapes expression and sequence of bacterial genes.Molecular cell · 2026Article
- GapR stiffens AT-rich and overtwisted DNA leading to differential effects on transcription based on gene context.Nucleic acids research · 2026Article
- BR-bodies link RNA homeostasis to stress tolerance and intracellular fitness inbioRxiv : the preprint server for biology · 2026Article
- Conserved 3' stem-loop structures enable comprehensive analysis of bacterial transcription termination in metagenomes.Microbiome · 2026Article
- Purine bias in bacterial genes is driven by runaway transcription.Nature microbiology · 2026Article
- Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.mSystems · 2026Article
- Structural insights into RNA recognition by theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Translation efficiency covariation identifies conserved coordination patterns across cell types.Nature biotechnology · 2026Article
- Revealing global stoichiometry conservation architecture in cells from Raman spectral patterns.eLife · 2026Article
- Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus.Nature communications · 2026Article
- A geometric deep learning framework for genome-wide prediction of enzyme turnover number.Genome biology · 2026Article
- Hybrid capture RNA-seq defines temporal gene expression inbioRxiv : the preprint server for biology · 2026Article
- Rif-seq reveals Caulobacter crescentus mRNA decay is globally coordinated with transcription and translation.Cell reports · 2025Article
- Article
- Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.bioRxiv : the preprint server for biology · 2025Article
- Considering Metabolic Context in Enzyme Evolution and Design.Biochemistry · 2025Review
- c-di-GMP-Dependent Regulation of Motility bybioRxiv : the preprint server for biology · 2025Article
56 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Coexpression of proteins in response to pathway-inducing signals is the founding paradigm of gene regulation. However, it remains unexplored whether the relative abundance of co-regulated proteins requires precise tuning. Here, we present large-scale analyses of protein stoichiometry and corresponding regulatory strategies for 21 pathways and 67-224 operons in divergent bacteria separated by 0.6-2 billion years. Using end-enriched RNA-sequencing (Rend-seq) with single-nucleotide resolution, we found that many bacterial gene clusters encoding conserved pathways have undergone massive divergence in transcript abundance and architectures via remodeling of internal promoters and terminators. Remarkably, these evolutionary changes are compensated post-transcriptionally to maintain preferred stoichiometry of protein synthesis rates. Even more strikingly, in eukaryotic budding yeast, functionally analogous proteins that arose independently from bacterial counterparts also evolved to convergent in-pathway expression. The broad requirement for exact protein stoichiometries despite regulatory divergence provides an unexpected principle for building biological pathways both in nature and for synthetic activities.
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