ArticleMethods in molecular biology (Clifton, N.J.)2018
Detecting Purinosome Metabolon Formation with Fluorescence Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- Coordination of cell organelles to promote metabolon formation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.Advances in experimental medicine and biology · 2026Review
- Phase separation of the PRPP amidotransferase into dynamic condensates promotes de novo purine synthesis in yeast.PLoS biology · 2025Article
- Purinosomes involved in the regulation of tumor metabolism: current progress and potential application targets.Frontiers in oncology · 2024Review
- The Purinosome: A Case Study for a Mammalian Metabolon.Annual review of biochemistry · 2022Review
- Human de novo purine biosynthesis.Critical reviews in biochemistry and molecular biology · 2021Review
- Hypoxia drives the assembly of the multienzyme purinosome complex.The Journal of biological chemistry · 2020Article
- Potential Mechanisms Connecting Purine Metabolism and Cancer Therapy.Frontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A long-standing hypothesis in the de novo purine biosynthetic pathway is that there must be highly coordinated processes to allow for enhanced metabolic flux when a cell demands purines. One mechanism by which the pathway meets its cellular demand is through the spatial organization of pathway enzymes into multienzyme complexes called purinosomes. Cellular conditions known to impact the activity of enzymes in the pathway or overall pathway flux have been reflected in a change in the number of purinosome-positive cells or the density of purinosomes in a given cell. The following general protocols outline the steps needed for purinosome detection through transient expression of fluorescent protein chimeras or through immunofluorescence in purine-depleted HeLa cells using confocal laser scanning microscopy. These protocols define a purinosome as a colocalization of FGAMS with one additional pathway enzyme, such as PPAT or GART, and provide insights into the proper identification of a purinosome from other reported cellular bodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.