Evidence map›Paper›PMID 29605920›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI.

Fabian Hertel, Gary C H Mo, Peter Dedecker, Jin Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Fabian HertelDepartment of Chemistry, University of Leuven, Heverlee, Belgium.
Gary C H MoDepartment of Pharmacology, University of California at San Diego, La Jolla, CA, USA.
Peter DedeckerDepartment of Chemistry, University of Leuven, Heverlee, Belgium.
Jin ZhangDepartment of Pharmacology, University of California at San Diego, La Jolla, CA, USA. jzhang32@ucsd.edu.

Funding

Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2M
Mechanisms of Compartmentalized cAMP SignalingR01DK073368 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2006 to 2026
$7.6M
Multiplex in vivo imaging of cell-specific and circuit-specific signaling pathways during synaptic plasticityR01MH111516 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L, ZHANG, JIN · 2016 to 2018
$1.8M
Signal Transduction by PI3K/Akt/mTOR PathwayR01GM111665 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHANG, JIN · 2015 to 2018
$1.2M
NCI NIH HHS R35 CA197622NIDDK NIH HHS R01 DK073368NIGMS NIH HHS R01 GM111665NIMH NIH HHS R01 MH111516
6 · The paper itself

Abstract

Few approaches are currently available that allow the detection of protein-protein interactions (PPIs) in super-resolution, and the observation of the assembly of protein complexes in living cells has been particularly challenging. We developed reconstituted fluorescence-based stochastic optical fluctuation imaging (refSOFI), which is based on bimolecular fluorescence complementation (BiFC) and SOFI, allowing us to detect protein complex assembly 30 min after the induction of complex formation. Here we describe how to use refSOFI to map the assembly of two proteins of interest into a complex within living cells at super-resolution.

Indexed as

FluorescenceProtein Interaction Domains and MotifsHeLa CellsHumansMicroscopy, FluorescenceNeoplasm ProteinsOptical ImagingORAI1 ProteinStromal Interaction Molecule 1Neoplasm ProteinsORAI1 ProteinORAI1 protein, humanSTIM1 protein, humanStromal Interaction Molecule 1Bimolecular fluorescence complementation (BiFC)Protein complexesProtein-protein interactionsStochastic optical fluctuation imaging (SOFI)Super-resolution imaging

Identifiers

PMID29605920
PMCPMC8580746

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.