ArticleMethods in molecular biology (Clifton, N.J.)2018
Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 6 citations in OpenAlex.
- High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials.Chembiochem : a European journal of chemical biology · 2022Article
- Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy.Nanoscale · 2021Article
- Microchip-Based Structure Determination of Disease-Relevant p53.Analytical chemistry · 2020Article
- Cryo-EM-On-a-Chip: Custom-Designed Substrates for the 3D Analysis of Macromolecules.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Challenges and opportunities in cryo-EM single-particle analysis.The Journal of biological chemistry · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining monolayer purification with silicon nitride microchips to create a tunable approach for the EM community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.