Evidence map›Paper›PMID 29604476›Full record

ArticleVirology2018

Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2.

Ronald L Veselenak, Gregg N Milligan, Aaron L Miller, Richard B Pyles, Nigel Bourne

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ronald L VeselenakDepartment of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555. Electronic address: rlvesele@utmb.edu.
Gregg N MilliganDepartment of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555. Electronic address: gnmillig@utmb.edu.
Aaron L MillerDepartment of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555. Electronic address: aamiller@utmb.edu.
Richard B PylesDepartment of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555. Electronic address: rbpyles@utmb.edu.
Nigel BourneDepartment of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555. Electronic address: nibourne@utmb.edu.
The University of Texas Medical Branch at Galveston · US

Funding

Induction, maintenance, and function of genital tract-resident CD8+ T cellsR01AI107784 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI MILLIGAN, GREGG N. · 2015 to 2019
$1.7M
Therapeutic immunization to impact HSV-2 latency and associated pathogenesisR21AI105962 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI BOURNE, NIGEL · 2013 to 2014
$414k
Molecular Analyses of MG Pathogenesis in Human Cell Culture and Mouse ModelsR56AI087768 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI PYLES, RICHARD B · 2010 to 2010
$383k
NIAID NIH HHS R01 AI107784NIAID NIH HHS R21 AI105962NIAID NIH HHS R56 AI087768
6 · The paper itself

Abstract

Genital herpes infection in guinea pigs closely models human infection but tools for immune characterization are limited. Immunity to HSV infection at the vaginal epithelial surface was characterized in guinea pigs using PCR-based array analysis of vaginal swab samples. IFNγ was one of the most significantly upregulated genes throughout the infection and over 40% of genes with significantly altered expression were linked to IFNγ based on INTERFEROME analysis. IFNγ transcripts and biologically active IFNγ at the genital mucosa were confirmed by RTPCR and IFNγ reporter cells. Gene ontology analysis revealed activation of many biological processes related to genital immunity shared by humans and mice demonstrating the similarities of the local immune response to primary genital HSV-2 infection in guinea pigs and other established models. This transcription-based array will be useful for dissection of immunity during reactivation from latency, an infection outcome that is not well recapitulated by other animal models.

Indexed as

Host-Pathogen InteractionsImmunity, MucosalAnimalsDisease Models, AnimalGene Expression ProfilingGuinea PigsHerpes GenitalisHerpesvirus 2, HumanReverse Transcriptase Polymerase Chain Reactiongene expressionguinea pigHSV-2IFNγimmune responsemicroarrayvaginal mucosa

Identifiers

PMID29604476
PMCPMC5930930
OpenAlexW2794447186

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.