ArticleMicrobiologyOpen2018
Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria.
Article in MicrobiologyOpen, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 85 citations in OpenAlex.
- Multiple extracellular polymeric substance pathways transcribed byApplied and environmental microbiology · 2025Article
- A new strategy for greenhouse gas emission reduction in the anaerobic/anoxic/oxic biological treatment process using exogenous N-acyl-homoserine lactones, a quorum-sensing signaling molecules.Water science and technology : a journal of the International Association on Water Pollution Research · 2024Article
- Extracellular polymeric substances as paper coating biomaterials derived from anaerobic granular sludge.Environmental science and ecotechnology · 2024Article
- Mechanisms of microbial co-aggregation in mixed anaerobic cultures.Applied microbiology and biotechnology · 2024Review
- Aerobic granular sludge for complex heavy metal-containing wastewater treatment: characterization, performance, and mechanisms analysis.Frontiers in microbiology · 2024Article
- Amyloid adhesin production in activated sludge is enhanced in lab-scale sequencing batch reactors: Feeding regime impacts microbial community and amyloid distribution.Water research X · 2022Article
- Amyloid Aggregation of Streptococcus mutans Cnm Influences Its Collagen-Binding Activity.Applied and environmental microbiology · 2021Article
- Decorating the Anammox House: Sialic Acids and Sulfated Glycosaminoglycans in the Extracellular Polymeric Substances of Anammox Granular Sludge.Environmental science & technology · 2020Article
- Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria.MicrobiologyOpen · 2018Article
- Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.Environmental science & technology · 2018Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different glycoconjugates. Its intrinsic fluorescence was captured to visualize the location of the extracted EPS in the nitrifying granules, and its hydrogel-forming property was studied by rheometry. The extracted EPS is abundant with cross ß-sheet secondary structure, contains glycosylated proteins/polypeptides, and rich in tryptophan. It forms hydrogel with high mechanical strength. The extraction and discovery of glycosylated proteins and/or amyloids further shows that conventionally used extraction and characterization techniques are not adequate for the study of structural extracellular polymers in biofilms and/or granular sludge. Confirming amyloids secondary structure in such a complex sample is challengeable due to the possibility of amyloids glycosylation and self-assembly. A new definition of extracellular polymers components which includes glycosylated proteins and a better approach to studying them is required to stimulate biofilm research.
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