ArticleJournal of the American Society for Mass Spectrometry2018
Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS.
Article in Journal of the American Society for Mass Spectrometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 116 citations in OpenAlex.
- Navigating the glycomics landscape with CE-MS: advances in sample preparation and analytical strategies.The Analyst · 2026Review
- N-glycopedia: libraries for native N-glycan structural analysis.Nature communications · 2026Article
- Isomeric diversity of GlcNAc(ManScientific reports · 2026Article
- A novel glycoform of the rotavirus outer capsid protein VP7 is secreted from polarized cells and activates innate immune cells.Journal of virology · 2026Article
- Nanopore profiling and structure determination of oligosaccharides by fragmentation.Science advances · 2026Article
- dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms.Journal of proteome research · 2025Article
- Recent advances in analytical methods and bioinformatic tools for quantitative glycomics.Analytical and bioanalytical chemistry · 2025Review
- Navigating the maze of mass spectra: a machine-learning guide to identifying diagnostic ions in O-glycan analysis.Analytical and bioanalytical chemistry · 2025Article
- Exploring the O-glycomic Degradome Using Natural Mucin Libraries.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Impact of different pathogen classes on the serumBBA advances · 2025Article
- Analysis of human biological samples using porous graphitic carbon columns and liquid chromatography-mass spectrometry: a review.Analytical and bioanalytical chemistry · 2024Review
- Deep learning method for the prediction of glycan structures from mass spectrometry data.Nature methods · 2024Article
- ComprehensiveAnalytical chemistry · 2024Article
- Multinozzle Emitter for Improved Negative Mode Analysis of Reduced NativeAnalytical chemistry · 2024Article
- Chromatograms and Mass Spectra of High-Mannose and PaucimannoseJournal of proteome research · 2024Article
- Mass Spectrometry Strategies forCells · 2024Review
- Quantitative subcellular reconstruction reveals a lipid mediated inter-organelle biogenesis network.Nature cell biology · 2024Article
- Study on biotransformation and absorption of genistin based on fecal microbiota and Caco-2 cell.Frontiers in pharmacology · 2024Article
- Inclusion of Porous Graphitic Carbon Chromatography Yields Greater Protein Identification and Compartment and Process Coverage and Enables More Reflective Protein-Level Label-Free Quantitation.Journal of proteome research · 2023Article
- Glycoproteome remodeling and organelle-specificProceedings of the National Academy of Sciences of the United States of America · 2023Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology. As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments. We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS. By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered. Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions. These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates. Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination. This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers. Graphical Abstract ᅟ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.