Evidence map›Paper›PMID 29601780›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2018

Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi.

Halcyon J Killion, William Edwards, Jessica Jennings-Gaines, Mary Wood, Karen Fox, Kerry Sondgeroth

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Halcyon J KillionWildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.
William EdwardsWildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.
Jessica Jennings-GainesWildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.
Mary WoodWildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.
Karen FoxWildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.
Kerry SondgerothWildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.
Colorado Parks and Wildlife · USUniversity of Wyoming · USWyoming Game and Fish Department · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A real-time PCR assay for the leukotoxin gene of Bibersteinia trehalosi was developed and validated to better identify this pathogen, which is a cause of respiratory disease in bighorn sheep. The specificity of the PCR primers was evaluated with DNA from 59 known isolates of the Pasteurellaceae family. For validation, 162 field samples were compared using both the new assay and an indirect method using 2 sets of published protocols. The real-time PCR assay was found to be specific for the leukotoxin gene of B. trehalosi and provides a rapid and direct approach for detecting leukotoxin-producing forms of this organism from samples containing mixed species of leukotoxin-positive Pasteurellaceae.

Indexed as

Real-Time Polymerase Chain ReactionSheep, BighornAnimalsBacterial InfectionsExotoxinsGammaproteobacteriaRespiratory Tract InfectionsExotoxinsleukotoxinBibersteinia trehalosibighorn sheepleukotoxinOvis canadensisPCR

Identifiers

PMID29601780
PMCPMC6505900
OpenAlexW2794645308

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.