Evidence map›Paper›PMID 29601110›Full record

ArticleJournal of leukocyte biology2018

Expression of novel "LOCGEF" isoforms of ARHGEF18 in eosinophils.

Keren B Turton, Emily M Wilkerson, Alex S Hebert, Frances J Fogerty, Hazel M Schira, Fady E Botros, Joshua J Coon, Deane F Mosher

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of leukocyte biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. GPCRs thatSmall GTPases · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Keren B TurtonDepartment of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Emily M WilkersonDepartment of Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Alex S HebertDepartment of Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Frances J FogertyDepartment of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Hazel M SchiraDepartment of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Fady E BotrosDepartment of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Joshua J CoonDepartment of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
Deane F MosherDepartment of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
University of Wisconsin–Madison · US

Funding

Thr Role of Pin1 in AirwayP01HL088594 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI JARJOUR, NIZAR N · 2008 to 2017
$21.0M
RESEARCH TRAINING IN HEMATOLOGYT32HL007899 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI Jane Ellen Churpek · 1998 to 2026
$8.2M
Proteomics of eosinophil activationR01AI125390 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J, MOSHER, DEANE FREMONT · 2016 to 2019
$1.5M
NHLBI NIH HHS P01 HL088594NHLBI NIH HHS T32 HL007899NIAID NIH HHS R01 AI125390
6 · The paper itself

Abstract

Genomic, transcriptomic and proteomic databases indicate that the N-terminal 322 residues encoded by the presumptive LOC100996504 gene, which is adjacent to the ARHGEF18 guanine nucleotide exchange factor gene on chromosome 19, constitute the N-terminal portion of a 1361-residue isoform of ARHGEF18, dubbed LOCGEF-X3. LOCGEF-X3 arises from the use of a leukocyte-specific alternative transcriptional start site and splicing that bypasses the initial noncoding exon of the canonical 1015-residue ARHGEF18 isoform, p114. Eosinophil LOCGEF-X3 was amplified and cloned, recombinant LOCGEF-X3 was expressed, and anti-ARHGEF18 antibody was found to recognize a band in immunoblots of eosinophil lysates that co-migrates with recombinant LOCGEF-X3. PCR of eosinophils revealed minor amounts of transcripts for X4 and X5 isoforms of LOCGEF that arise from differential splicing and differ from the X3 isoform at their extreme N-termini. No p114 transcript or protein band was detected in eosinophils. Immunostaining with anti-ARHGEF18 antibody revealed relocalization of LOCGEF and RHOA from the periphery of round unstimulated eosinophils to the 2 poles of eosinophils polarized by treatment with IL5, CCL11, or IL33 in suspension. Canonical p114 ARHGEF18 has been implicated in maintenance of epithelial cell polarity. We suggest that the "LOC" portion of LOCGEF, which is unlike any other protein domain, has unique functions in control of polarity in activated eosinophils and other leukocytes.

Indexed as

Cell PolarityEosinophilsHumansProtein IsoformsProteomicsRho Guanine Nucleotide Exchange FactorsARHGEF18 protein, humanProtein IsoformsRho Guanine Nucleotide Exchange Factorsalternative transcriptional startARHGEF18granulocyteguanine nucleotide exchange factorLOC100996504p114-GEF

Identifiers

PMID29601110
PMCPMC6013370
OpenAlexW2794831601

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.