ArticleFrontiers in plant science2018
Activation of ROP6 GTPase by Phosphatidylglycerol in Arabidopsis.
Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Ustilago maydis Phosphatidic Acid and Phosphatidylserine-Targeting Secreted Lipase Lip3 Is Involved in Pathogenesis.Molecular plant pathology · 2026Article
- Exploring lipid-protein interactions in plant membranes.Journal of experimental botany · 2024Review
- Phosphatidic acid-regulated SOS2 controls sodium and potassium homeostasis in Arabidopsis under salt stress.The EMBO journal · 2023Article
- CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in rice.The Plant journal : for cell and molecular biology · 2022Article
- Genome-Wide Identification of Genes Encoding for Rho-Related Proteins in 'Plants (Basel, Switzerland) · 2022Article
- Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations.International journal of molecular sciences · 2022Review
- Subcellular trafficking and post-translational modification regulate PIN polarity in plants.Frontiers in plant science · 2022Review
- Phospholipids in Salt Stress Response.Plants (Basel, Switzerland) · 2021Review
- No Time for Transcription-Rapid Auxin Responses in Plants.Cold Spring Harbor perspectives in biology · 2021Review
- Review
- An improved protein lipid overlay assay for studying lipid-protein interactions.Plant methods · 2020Article
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant Rho-like GTPases (ROPs) are switch-like proteins which play essential roles in controlling cell polarity development and cellular activities. ROPs are regulated by many factors, such as auxin, light, and RopGEFs and RopGAPs proteins. However, it has not been reported yet whether small molecules play a role in the regulation of ROP activity. Here, we showed that AtROP6 specially bound to a phospholipid, phosphatidylglycerol (PG), by the protein-lipid overlay and liposome sedimentation assays, and further MST assay gave a dissociation constant (Kd) of 4.8 ± 0.4 μM for binding of PG to His-AtROP6. PG profile analysis in Arabidopsis revealed that PG existed both in leaves and roots but with distinctive fatty acyl chain patterns. By evaluating AtROP6 activity using RIC1 effector binding-based assay, we found that PG stimulated AtROP6 activity. In the FM4-64 uptake experiment, PG inhibited AtROP6-mediated endocytosis process. By evaluating internalization of PIN2, PG was shown to regulate endocytosis process coordinately with NAA. Further root gravitropism experiment revealed that PG enhanced the AtROP6-mediated root gravity response. These results suggest that the phospholipid PG physically binds AtROP6, stimulates its activity and influences AtROP6-mediated root gravity response in Arabidopsis.
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