ArticleThe ISME journal2018
Cultivation and genomics of the first freshwater SAR11 (LD12) isolate.
Article in The ISME journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 194 citations in OpenAlex.
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- Metagenome-based vertical profiling of the Gulf of Mexico highlights its uniqueness and far-reaching effects of freshwater input.Applied and environmental microbiology · 2026Article
- Cell cycle dysregulation of globally important SAR11 bacteria resulting from environmental perturbation.Nature microbiology · 2026Article
- Metabolic Potential of Candidatus Saccharimonadia Including Rare Lineages in Activated Sludge.Environmental microbiology reports · 2025Article
- Detergent-based separation of microbes from marine particles.Applied and environmental microbiology · 2025Article
- Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Widely-distributed freshwater microorganisms with streamlined genomes co-occur in cohorts with high abundance.Scientific reports · 2025Article
- Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota.Nature microbiology · 2025Article
- Ecophysiology and global dispersal of the freshwater SAR11-IIIb genus Fontibacterium.Nature microbiology · 2025Article
- Bringing the uncultivated microbial majority of freshwater ecosystems into culture.Nature communications · 2025Article
- Scalable genotyping of microbial colonies.Microbial genomics · 2025Article
- Extensive paralogism in the environmental pangenome: a key factor in the ecological success of natural SAR11 populations.Microbiome · 2025Article
- Culture-supported ecophysiology of the SAR116 clade demonstrates metabolic and spatial niche partitioning.The ISME journal · 2025Article
- Polyhydroxybutyrate production by freshwater SAR11 (LD12).The ISME journal · 2025Article
- New isolates refine the ecophysiology of the Roseobacter CHAB-I-5 lineage.ISME communications · 2025Article
- Impact of growth conditions on the abundance and diversity of cultivable bacteria recovered fromFEMS microbes · 2025Article
- Contrasting defense strategies of oligotrophs and copiotrophs revealed by single-cell-resolved virus-host pairing of freshwater bacteria.ISME communications · 2025Article
- Shaping the future of tobacco through microbial insights: a review of advances and applications.Frontiers in bioengineering and biotechnology · 2025Review
- Bacterial Communities From Two Freshwater Aquaculture Systems in Northern Germany.Environmental microbiology reports · 2024Article
- Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evolutionary transitions between fresh and salt water happen infrequently among bacterioplankton. Within the ubiquitous and highly abundant heterotrophic Alphaproteobacteria order Pelagibacterales (SAR11), most members live in marine habitats, but the LD12 subclade has evolved as a unique freshwater lineage. LD12 cells occur as some of the most dominant freshwater bacterioplankton, yet this group has remained elusive to cultivation, hampering a more thorough understanding of its biology. Here, we report the first successful isolation of an LD12 representative, strain LSUCC0530, using high-throughput dilution-to-extinction cultivation methods, and its complete genome sequence. Growth experiments corroborate ecological data suggesting active populations of LD12 in brackish water up to salinities of ~5. LSUCC0530 has the smallest closed genome thus far reported for a SAR11 strain (1.16 Mbp). The genome affirms many previous metabolic predictions from cultivation-independent analyses, like a complete Embden-Meyerhof-Parnas glycolysis pathway, but also provides novel insights, such as the first isocitrate dehydrogenase in LD12, a likely homologous recombination of malate synthase from outside of the SAR11 clade, and analogous substitutions of ion transporters with others that occur throughout the rest of the SAR11 clade. Growth data support metagenomic recruitment results suggesting temperature-based ecotype diversification within LD12. Key gene losses for osmolyte uptake provide a succinct hypothesis for the evolutionary transition of LD12 from salt to freshwater. For strain LSUCC0530, we propose the provisional nomenclature Candidatus fonsibacter ubiquis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.