ArticleNature protocols2018
Fiber-optic implant for simultaneous fluorescence-based calcium recordings and BOLD fMRI in mice.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Neuroimaging of the effects of drug exposure or self-administration in rodents: A systematic review.Neuroscience and biobehavioral reviews · 2024Pooled it
- Simultaneous single-cell calcium imaging of neuronal population activity and brain-wide BOLD fMRI.Nature methods · 2026Article
- Non-invasive large-scale imaging of concurrent neuronal, astrocytic, and hemodynamic activity with hybrid multiplexed fluorescence and magnetic resonance imaging (HyFMRI).Light, science & applications · 2025Article
- Neuropeptide Y neurons mediate opioid-induced itch by disinhibiting GRP-GRPR microcircuits in the spinal cord.Nature communications · 2025Article
- Systematic evaluation of adhesives for implant fixation in multimodal functional brain MRI.Magma (New York, N.Y.) · 2025Article
- A standardized image processing and data quality platform for rodent fMRI.Nature communications · 2024Article
- Review
- Translational In Vivo Assays in Behavioral Biology.Annual review of pharmacology and toxicology · 2024Review
- Moderate white light exposure enhanced spatial memory retrieval by activating a central amygdala-involved circuit in mice.Communications biology · 2023Article
- Monitoring mouse brain perfusion with hybrid magnetic resonance optoacoustic tomography.Biomedical optics express · 2023Article
- Generation of a whole-brain hemodynamic response function and sex-specific differences in cerebral processing of mechano-sensation in mice detected by BOLD fMRI.Frontiers in neuroscience · 2023Article
- GRIN lens applications for studying neurobiology of substance use disorder.Addiction neuroscience · 2022Article
- Individual slow wave events give rise to macroscopic fMRI signatures and drive the strength of the BOLD signal in human resting-state EEG-fMRI recordings.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- Dynamic reorganization of the cortico-basal ganglia-thalamo-cortical network during task learning.Cell reports · 2022Article
- Spectral fiber photometry derives hemoglobin concentration changes for accurate measurement of fluorescent sensor activity.Cell reports methods · 2022Article
- Fiber-based lactate recordings with fluorescence resonance energy transfer sensors by applying an magnetic resonance-informed correction of hemodynamic artifacts.Neurophotonics · 2022Article
- Toward an integrative neurovascular framework for studying brain networks.Neurophotonics · 2022Article
- Building bridges: simultaneous multimodal neuroimaging approaches for exploring the organization of brain networks.Neurophotonics · 2022Review
- Hybrid fiber optic-fMRI for multimodal cell-specific recording and manipulation of neural activity in rodents.Neurophotonics · 2022Review
- Spatiotemporal patterns of spontaneous brain activity: a mini-review.Neurophotonics · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the growing popularity of blood oxygen level-dependent (BOLD) functional MRI (fMRI), understanding of its underlying principles is still limited. This protocol describes a technique for simultaneous measurement of neural activity using fluorescent calcium indicators together with the corresponding hemodynamic BOLD fMRI response in the mouse brain. Our early work using small-molecule fluorophores in rats gave encouraging results but was limited to acute measurements using synthetic dyes. Our latest procedure combines fMRI with optical detection of cell-type-specific virally delivered GCaMP6, a genetically encoded calcium indicator (GECI). GCaMP6 fluorescence, which increases upon calcium binding, is collected by a chronically implanted optical fiber, allowing longitudinal studies in mice. The chronic implant, placed horizontally on the skull, has an angulated tip that reflects light into the brain and is connected via fiber optics to a remote optical setup. The technique allows access to the neocortex and does not require adaptations of commercial MRI hardware. The hybrid approach permits fiber-optic calcium recordings with simultaneous artifact-free BOLD fMRI with full brain coverage and 1-s temporal resolution using standard gradient-echo echo-planar imaging (GE-EPI) sequences. The method provides robust, cell-type-specific readouts to link neural activity to BOLD signals, as emonstrated for task-free ('resting-state') conditions and in response to hind-paw stimulation. These results highlight the power of fiber photometry combined with fMRI, which we aim to further advance in this protocol. The approach can be easily adapted to study other molecular processes using suitable fluorescent indicators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.