ArticleThe Journal of cell biology1987
Direction of force generated by the inner row of dynein arms on flagellar microtubules.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 81 citations in OpenAlex.
- DRC2/CCDC65 is a central hub for assembly of the nexin-dynein regulatory complex and other regulators of ciliary and flagellar motility.Molecular biology of the cell · 2018Article
- Cilia in Left-Right Symmetry Breaking.Cold Spring Harbor perspectives in biology · 2017Review
- A computational model of dynein activation patterns that can explain nodal cilia rotation.Biophysical journal · 2015Article
- The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes.Molecular biology of the cell · 2013Article
- Regulation of ciliary motility: conserved protein kinases and phosphatases are targeted and anchored in the ciliary axoneme.Archives of biochemistry and biophysics · 2011Review
- A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product.The Journal of cell biology · 2003Article
- A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20 associates with Spag6, the mammalian orthologue of Chlamydomonas PF16.Molecular and cellular biology · 2002Article
- The sup-pf-2 mutations of Chlamydomonas alter the activity of the outer dynein arms by modification of the gamma-dynein heavy chain.The Journal of cell biology · 1996Article
- The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes.Molecular biology of the cell · 1995Article
- Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella.The Journal of cell biology · 1994Article
- The bop2-1 mutation reveals radial asymmetry in the inner dynein arm region of Chlamydomonas reinhardtii.The Journal of cell biology · 1994Article
- The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond.The Journal of cell biology · 1992Article
- The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella.The Journal of cell biology · 1992Article
- The response of the Golgi complex to microtubule alterations: the roles of metabolic energy and membrane traffic in Golgi complex organization.The Journal of cell biology · 1989Article
- Isolated beta-heavy chain subunit of dynein translocates microtubules in vitro.The Journal of cell biology · 1988Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Our goal was to determine the direction of force generation of the inner dynein arms in flagellar axonemes. We developed an efficient means of extracting the outer row of dynein arms in demembranated sperm tail axonemes, leaving the inner row of dynein arms structurally and functionally intact. Sperm tail axonemes depleted of outer arms beat at half the beat frequency of sperm tails with intact arms over a wide range of ATP concentrations. The isolated, outer arm-depleted axonemes were induced to undergo microtubule sliding in the presence of ATP and trypsin. Electron microscopic analysis of the relative direction of microtubule sliding (see Sale, W. S. and P. Satir, 1977, Proc. Natl. Acad. Sci. USA, 74:2045-2049) revealed that the doublet microtubule with the row of inner dynein arms, doublet N, always moved by sliding toward the proximal end of the axoneme relative to doublet N + 1. Therefore, the inner arms generate force such that doublet N pushes doublet N + 1 tipward. This is the same direction of microtubule sliding induced by ATP and trypsin in axonemes having both inner and outer dynein arms. The implications of this result for the mechanism of ciliary bending and utility in functional definition of cytoplasmic dyneins are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.