ArticleNucleic acids research2018
Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Mismatch type impacts interference and priming activities in the type I-E CRISPR-Cas system.The Journal of biological chemistry · 2026Article
- TAV2b Peptide Derivatives Underwind and Stabilize Double-Stranded RNA upon Binding.Journal of the American Chemical Society · 2026Article
- Mismatch type impacts interference and priming activities in the type I-E CRISPR-Cas system.bioRxiv : the preprint server for biology · 2026Article
- TheEcoSal Plus · 2025Review
- Continuous targeted hypermutation with a tunable mutation window.Nature communications · 2025Article
- A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex.Nature communications · 2022Article
- Creating memories: molecular mechanisms of CRISPR adaptation.Trends in biochemical sciences · 2022Review
- The phylogenomics of CRISPR-Cas system and revelation of its features in Salmonella.Scientific reports · 2020Article
- Primed CRISPR DNA uptake in Pyrococcus furiosus.Nucleic acids research · 2020Article
- Detection of CRISPR adaptation.Biochemical Society transactions · 2020Review
- CRISPR DNA elements controlling site-specific spacer integration and proper repeat length by a Type II CRISPR-Cas system.Nucleic acids research · 2019Article
- Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length.Cell reports · 2019Article
- Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas.Molecular cell · 2019Article
- Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.Molecular microbiology · 2019Article
- Mechanisms of Type I-E and I-F CRISPR-Cas Systems inEcoSal Plus · 2019Review
- Dynamics of Cas10 Govern Discrimination between Self and Non-self in Type III CRISPR-Cas Immunity.Molecular cell · 2019Article
- Article
- Role of nucleotide identity in effective CRISPR target escape mutations.Nucleic acids research · 2018Article
- CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases.Nucleic acids research · 2018Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
In type I CRISPR-Cas systems, primed adaptation of new spacers into CRISPR arrays occurs when the effector Cascade-crRNA complex recognizes imperfectly matched targets that are not subject to efficient CRISPR interference. Thus, primed adaptation allows cells to acquire additional protection against mobile genetic elements that managed to escape interference. Biochemical and biophysical studies suggested that Cascade-crRNA complexes formed on fully matching targets (subject to efficient interference) and on partially mismatched targets that promote primed adaption are structurally different. Here, we probed Escherichia coli Cascade-crRNA complexes bound to matched and mismatched DNA targets using a magnetic tweezers assay. Significant differences in complex stabilities were observed consistent with the presence of at least two distinct conformations. Surprisingly, in vivo analysis demonstrated that all mismatched targets stimulated robust primed adaptation irrespective of conformational states observed in vitro. Our results suggest that primed adaptation is a direct consequence of a reduced interference efficiency and/or rate and is not a consequence of distinct effector complex conformations on target DNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.