ArticleBiomedical microdevices2018
A sample-to-answer droplet magnetofluidic assay platform for quantitative methylation-specific PCR.
Article in Biomedical microdevices, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- First Decade of the National Cancer Institute's Affordable Cancer Technologies Program: Accelerating Translational Technology Research and Development for Cancer Globally.JCO global oncology · 2025Review
- Cancer Methylation Biomarker Detection in an Automated, Portable, Multichannel Magnetofluidic Platform.ACS nano · 2024Article
- The epigenetic modification of DNA methylation in neurological diseases.Frontiers in immunology · 2024Review
- Transfer of blocker-based qPCR reactions for DNA methylation analysis into a microfluidic LoC system using thermal modeling.Biomicrofluidics · 2022Article
- High-throughput sample processing for methylation analysis in an automated, enclosed environment.SLAS technology · 2022Article
- Droplet Manipulation under a Magnetic Field: A Review.Biosensors · 2022Review
- Droplet Magnetofluidic Assay Platform for Quantitative Methylation-Specific PCR.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Facile Coupling of Droplet Magnetofluidic-Enabled Automated Sample Preparation for Digital Nucleic Acid Amplification Testing and Analysis.Analytical chemistry · 2020Article
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Dysregulation of DNA methylation has been identified as an epigenetic biomarker for numerous cancer types. Gene-specific identification techniques relying on methylation-specific PCR (MSP) require a lengthy manual benchtop process that is susceptible to human-error and contamination. This MSP assay requires a series of discrete sample processing steps including genomic DNA extraction, bisulfite conversion and readout via PCR. In this work, we present a streamlined assay platform utilizing droplet magnetofluidic principles for integration of all sample processing steps required to obtain quantitative MSP signal from raw biological samples. We present a streamlined protocol for solid-phase extraction and bisulfite conversion of genomic DNA, which minimizes reagent use and simplifies the sample preparation protocol for implementation on a compact assay platform. Furthermore, we present a thermally robust assay chip that enables DNA extraction, bisulfite conversion and quantitative PCR from biological samples on a single device. Technical improvements to facilitate DNA extraction and PCR on a single chip in addition to chip performance characterization data are presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.