ArticleMikrochimica acta2018
A rapid colorimetric immunoassay for the detection of pathogenic bacteria on poultry processing plants using cotton swabs and nanobeads.
Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Photoelectrochemical Immuno-Sensing via Plasmon-Induced Resonance Energy Transfer Mechanism.ECS sensors plus · 2026Article
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- Target Recognition Triggered Split DNAzyme based Colorimetric Assay for Direct and Sensitive Methicillin-Resistance Analysis ofJournal of microbiology and biotechnology · 2024Article
- Review of Detection Limits for Various Techniques for Bacterial Detection in Food Samples.Nanomaterials (Basel, Switzerland) · 2024Review
- Oxygen Sensor-Based Respirometry and the Landscape of Microbial Testing Methods as Applicable to Food and Beverage Matrices.Sensors (Basel, Switzerland) · 2023Review
- Article
- A Colorimetric Immunosensor Based on Hemin@MI Nanozyme Composites, with Peroxidase-like Activity for Point-of-care Testing of Pathogenic E. coli O157:H7.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Development of rapid colorimetric assay for the detection of Influenza A and B viruses.Talanta · 2021Article
- Development of a Simple, Fast, and Cost-Effective Nanobased Immunoassay Method for Detecting Norovirus in Food Samples.ACS omega · 2020Article
- Multiplexed Immunosensors and Immunoarrays.Analytical chemistry · 2020Review
- A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles.Nanomaterials (Basel, Switzerland) · 2020Article
- Immunoassay for pathogenic bacteria using platinum nanoparticles and a hand-held hydrogen detector as transducer. Application to the detection of Escherichia coli O157:H7.Mikrochimica acta · 2019Article
- Novel epoxy-silica nanoparticles to develop non-enzymatic colorimetric probe for analytical immuno/bioassays.Analytica chimica acta · 2018Article
- Developments in Rapid Detection Methods for the Detection of FoodborneFrontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The work describes a simple cotton swab-based colorimetric immunoassay as a rapid screening tool for pathogenic bacteria on poultry processing plants. This immunosensing platform can be used for the detection of pathogens present on surfaces such as glass, stainless steel and chicken meat. Unlike the reported assays, here, cotton swab plays dual function: as a sample collector from the solid surfaces and as detection platform. The immunoassay was tested for the detection of 4 different bacteria; Salmonella typhimurium, Salmonella enteritidis, Staphylococcus aureus and Campylobacter jejuni. The immunoassays were fabricated by immobilizing specific antibody for each bacterium on a cotton swab that is used to recover the cells from contaminated surfaces. Then, a sandwich immunoassay was developed by immersing the cotton swab in different colored nanobead-conjugated antibody solutions which corresponds to different bacteria. The immunoassays response was detected colorimetrically by following the change in the color intensity produced by the nanobeads due to the specific binding on the cotton swab. This simple colorimetric assay is very sensitive with a detection limit of 10 cfu.mL
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