Evidence map›Paper›PMID 29594727›Full record

ArticleMikrochimica acta2018

Circular exponential amplification of photoinduced electron transfer using hairpin probes, G-quadruplex DNAzyme and silver nanocluster-labeled DNA for ultrasensitive fluorometric determination of pathogenic bacteria.

Xueqi Leng, Yu Wang, Rongguo Li, Su Liu, Jianzhuang Yao, Qianqian Pei, Xuejun Cui, Yuqin Tu, Dan Tang, Jiadong Huang

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Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xueqi LengCollege of Resources and Environment, University of Jinan, Jinan, 250022, People's Republic of China.
Yu WangCollege of Biological Sciences and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Rongguo LiJinan Maternity and Child Care Hospital, Jinan, 250022, People's Republic of China.
Su LiuCollege of Resources and Environment, University of Jinan, Jinan, 250022, People's Republic of China. stu_lius@ujn.edu.cn.
Jianzhuang YaoCollege of Biological Sciences and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Qianqian PeiKey Laboratory of Chemical Sensing & Analysis in Universities of Shandong, College of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Xuejun CuiKey Laboratory of Chemical Sensing & Analysis in Universities of Shandong, College of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Yuqin TuCollege of Resources and Environment, University of Jinan, Jinan, 250022, People's Republic of China.
Dan TangCollege of Biological Sciences and Technology, University of Jinan, Jinan, 250022, People's Republic of China.
Jiadong HuangCollege of Biological Sciences and Technology, University of Jinan, Jinan, 250022, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The authors describe a fluorometric strategy for the detection of pathogenic bacteria with ultrasensitivity and high specificity. This strategy relies on the combination of target-modulated photoinduced electron transfer (PET) between G-quadruplex DNAzyme and DNA (labeled with silver nanoclusters) along with hairpin probe-based circular exponential amplification. The reaction system involves three hairpin probes (H1, H2 and H3). Probe H1 contains an aptamer against S. Typhimurium and the recognition sequence for nicking endonuclease. It is used to recognize S. Typhimurium and participates in polymerase-catalyzed target recycle amplification and secondary-target recycle amplification. Probe H2 contains an aptamer against hemin and is used to form the G-quadruplex DNAzyme in the presence of hemin and potassium ion. It acts as the electron acceptor and quenches the fluorescence of the labeled DNA. Fluorescence is best measured at excitation/emission wavelengths of 567/650 nm. Probe H3 contains the template sequence for the synthesis of AgNCs and the H2-annealing sequence. Both H2 and H3 are utilized to perform a strand displacement reaction and to achieve PET between G-quadruplex DNAzyme and DNA/AgNCs. To the best of our knowledge, this is the first example of a PET between G-quadruplex DNAzyme and DNA/AgNCs coupled with circular exponential amplification. The assay has an ultra-low detection limit 8 cfu·mL

Indexed as

Aptamers, NucleotideBiosensing TechniquesDNADNA, CatalyticG-QuadruplexesInverted Repeat SequencesLimit of DetectionMetal NanoparticlesNucleic Acid Amplification TechniquesNucleic Acid HybridizationSalmonella typhimuriumSilverSpectrometry, FluorescenceAptamers, NucleotideDNADNA, CatalyticSilverBioanalysisBiosensorGel electrophoresisHairpinNicking endonucleasePolymeraseSignal amplificationStrand displacement amplificationS. Typhimurium

Identifiers

PMID29594727

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.