ArticleMikrochimica acta2017
Voltammetric sandwich immunoassay for Cronobacter sakazakii using a screen-printed carbon electrode modified with horseradish peroxidase, reduced graphene oxide, thionine and gold nanoparticles.
Article in Mikrochimica acta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 17 citations in OpenAlex.
- Review
- High-throughput photoelectrochemical determination of E. coli O157:H7 by modulation of the anodic photoelectrochemistry of CdS quantum dots via reversible deposition of MnOMikrochimica acta · 2019Article
- Signal amplification in immunoassays by using noble metal nanoparticles: a review.Mikrochimica acta · 2019Review
- Colorimetric evaluation of the hydroxyl radical scavenging ability of antioxidants using carbon-confined CoOMikrochimica acta · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The authors describe a sandwich-type of electrochemical immunoassay for rapid determination of the foodborne pathogen Cronobacter sakazakii (C. sakazakii). Polyclonal antibody against C. sakazakii (anti-C. sakazakii) and horseradish peroxidase were immobilized on a nanocomposite consisting of reduced graphene oxide, thionine and gold nanoparticles (AuNPs) that was placed on a screen-printed carbon electrode (SPCE). Thionine acts as an electron mediator which also shortens the electron transfer pathway from the conjugated HRP to the electrode surface and amplifies the electrochemical signal. The AuNPs, in turn, improve the electron transfer rate and increase the surface area for capturing antibody. The morphologies of the electrodes were characterized by means of field emission scanning electron microscopy. The electrochemical performance of the immunoassay was evaluated by cyclic voltammetry and differential pulse voltammetry. Under optimal experimental conditions, the electrochemical immunoassay, best operated at a woking potential of -0.18 V (vs. Ag/AgCl) and scan rate of 20 mV/s has a linear response that covers the 8.8 × 10
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