ArticleMikrochimica acta2017
Colorimetric theophylline aggregation assay using an RNA aptamer and non-crosslinking gold nanoparticles.
Article in Mikrochimica acta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Perspectives in Aptasensor-Based Portable Detection for Biotoxins.Molecules (Basel, Switzerland) · 2024Review
- Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications.Molecules (Basel, Switzerland) · 2023Review
- Identification and ultrasensitive photoelectrochemical detection of LncNR_040117: a biomarker of recurrent miscarriage and antiphospholipid antibody syndrome in platelet-derived microparticles.Journal of nanobiotechnology · 2022Article
- DNA-functionalized gold nanoparticles: Modification, characterization, and biomedical applications.Frontiers in chemistry · 2022Review
- Nano optical and electrochemical sensors and biosensors for detection of narrow therapeutic index drugs.Mikrochimica acta · 2021Review
- High Stability Au NPs: From Design to Application in Nanomedicine.International journal of nanomedicine · 2021Review
- Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors.Nucleic acids research · 2020Review
- Electrochemical fabrication of reduced MoSMikrochimica acta · 2020Article
- A multi-channel localized surface plasmon resonance system for absorptiometric determination of abscisic acid by using gold nanoparticles functionalized with a polyadenine-tailed aptamer.Mikrochimica acta · 2019Article
- Carbon nanoparticle-protected RNA aptasensor for amplified fluorescent determination of theophylline in serum based on nuclease-aided signal amplification.RSC advances · 2019Article
- A fluorometric and colorimetric method for determination of trypsin by exploiting the gold nanocluster-induced aggregation of hemoglobin-coated gold nanoparticles.Mikrochimica acta · 2019Article
- Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.Mikrochimica acta · 2019Article
- Colorimetric sensing strategy for heparin assay based on PDDA-induced aggregation of gold nanoparticles.Nanoscale advances · 2019Article
- Application of Gold-Nanoparticle Colorimetric Sensing to Rapid Food Safety Screening.Sensors (Basel, Switzerland) · 2018Review
- Colorimetric and visual determination of acrylamide via acrylamide-mediated polymerization of acrylamide-functionalized gold nanoparticles.Mikrochimica acta · 2018Article
- Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticles.Mikrochimica acta · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The authors are presenting a rapid method for the determination of theophylline using unique non-crosslinking gold nanoparticle (AuNP) aggregation. An RNA aptamer against theophylline is firstly split into two RNA fragments which then interact with bare AuNPs. The two RNA probes cause an enhancement of the salt tolerance of AuNPs. However, in the presence of theophylline, the RNA probes form a complex with theophylline so that less RNA probes are available to protect the AuNPs from salt-induced aggregation. Theophylline induced aggregation of AuNPs is accompanied by a color change from red to blue. The color change can be detected visually and via UV-vis absorptiometry by ratioing the absorbances at 650 and 520 nm. The ratio increases linearly in the 0.1 to 20 μM theophylline concentration range, with a 67 nM limit of detection. The method is highly sensitive and selective. Graphical abstract Single-stranded split RNA aptamers (R1 and R2) protect gold nanoparticles (AuNPs) from salt-induced non-crosslinking aggregation. After recognition of theophylline by the RNA probe, the unprotected AuNPs aggregate and undergo a color change from red to blue, and this is used to quantify the theophylline concentration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.