Evidence map›Paper›PMID 29594617›Full record

ArticleMikrochimica acta2018

Fluorescence polarization gene assay for HIV-DNA based on the use of dendrite-modified gold nanoparticles acting as signal amplifiers.

Shuping Liang, Guicheng He, Jianniao Tian, Yanchun Zhao, Shulin Zhao

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
  3. Nanomaterials Used in Fluorescence Polarization Based Biosensors.International journal of molecular sciences · 2022
    Review
  4. Review
  5. Biomedical applications of nanoflares: Targeted intracellular fluorescence probes.Nanomedicine : nanotechnology, biology, and medicine · 2019
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shuping LiangState Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, China.
Guicheng HeState Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, China.
Jianniao TianState Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, China. birdtjn@sina.com.
Yanchun ZhaoState Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, China. yanchunzhao@aliyun.com.
Shulin ZhaoState Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, China.

Funding

National Natural Science Foundation of China 21165004, 21163002, 21465007, 21565007
6 · The paper itself

Abstract

The authors describe a fluorescence polarization assay for HIV-DNA. It is based on the use of gold nanoparticles (AuNPs) modified with DNA dendritic macromolecules that act as signal amplifiers. In the presence of HIV-DNA, the AuNP-DNA dendritic macromolecules and fluorescently labeled DNA probe combine with HIV-DNA in a sandwich format to form a conjugate. This reaction slows down the rotational speed of the labeled DNA probe because of the increase of molecular weight and volume. This increases fluorescence polarization and the sensitivity of the system. The relative fluorescence polarization values increase linearly in the 150 pM to 6 nM HIV-DNA concentration range, with a 73 pM detection limit. The results show this amplification strategy to be most useful for ultrasensitive determination of oligonucleotides by means of fluorescence polarization. Graphical abstract Schematic of a novel fluorescence polarization assay for the HIV-DNA. Ultrasensitive detection is accomplished by using AuNP-DNA dendritic macromolecules as signal amplification factor.

Indexed as

DendrimersDNA ProbesDNA, ViralFluorescence PolarizationGoldHIVHumansMetal NanoparticlesDendrimersDNA ProbesDNA, ViralGoldDendritic macromolecules self-assembly of gold nanoparticlesFluorescence polarizationSandwich hybridization compound

Identifiers

PMID29594617

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.