Evidence map›Paper›PMID 29594560›Full record

ArticleWorld journal of microbiology & biotechnology2018

Rate-limiting steps in the Saccharomyces cerevisiae ergosterol pathway: towards improved ergosta-5,7-dien-3β-ol accumulation by metabolic engineering.

Bin-Xiang Ma, Xia Ke, Xiao-Ling Tang, Ren-Chao Zheng, Yu-Guo Zheng

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Recent progress in strategies for steroid production in yeasts.World journal of microbiology & biotechnology · 2022
    Review
  7. Review
  8. Article
  9. Yeast as a promising heterologous host for steroid bioproduction.Journal of industrial microbiology & biotechnology · 2020
    Review
  10. Article
  11. RNA-Seq de Novo Assembly and Differential Transcriptome Analysis of Chaga (International journal of molecular sciences · 2019
    Article
  12. Article
  13. Outline of the biosynthesis and regulation of ergosterol in yeast.World journal of microbiology & biotechnology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Bin-Xiang MaKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Xia KeKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Xiao-Ling TangKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Ren-Chao ZhengKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
Yu-Guo ZhengKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China. zhengyg@zjut.edu.cn.
Zhejiang University of Technology · CN

Funding

National Natural Science Foundation of China 31400978Natural Science Foundation of Zhejiang Province LY18B020019
6 · The paper itself

Abstract

Ergosterol is the predominant nature sterol constituent of plasma membrane in Saccharomyces cerevisiae. Herein, the biosynthetic pathway of ergosterol was proposed to be metabolically engineered for the efficient production of ergosta-5,7-dien-3β-ol, which is the precursor of vitamin D4. By target disruption of erg5, involved in the end-steps of post-squalene formation, predominantly accumulated ergosta-5,7-dien-3β-ol (4.12 mg/g dry cell weight). Moreover, the rate-limiting enzymes of ergosta-5,7-dien-3β-ol biosynthesis were characterized. Overexpression of Hmg1p led to a significant accumulation of squalene, and induction of Erg1p/Erg11p expression raised the yield of both total sterols and ergosta-5,7-dien-3β-ol with no obvious changes in growth behavior. Furthermore, the transcription factor allele upc2-1 was overexpressed to explore the effect of combined induction of rate-limiting enzymes. Compared with an obviously enhanced yield of ergosterol in the wild-type strain, decreases of both the ergosta-5,7-dienol levels and the total sterol yield were found in Δerg5-upc2-1, probably due to the unbalanced NADH/NAD

Indexed as

Metabolic EngineeringBiomassCloning, MolecularCytochrome P-450 Enzyme SystemErgosterolGene Expression Regulation, FungalGene Knockout TechniquesMetabolic Networks and PathwaysNADSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSqualeneSterolsTime FactorsTrans-ActivatorsTranscription Factors22,23-dihydroergosterolCytochrome P-450 Enzyme SystemErg11 protein, S cerevisiaeERG5 protein, S cerevisiaeErgosterolNADSaccharomyces cerevisiae ProteinsSqualeneSterolsTrans-ActivatorsTranscription FactorsUPC2 protein, S cerevisiaeErgosta-5,7-dien-3β-olErgosterol synthesis pathwayRate-limiting stepsSaccharomyces cerevisiaeUpc2-1

Identifiers

PMID29594560
OpenAlexW2795145422

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.