ArticleMikrochimica acta2017
Fluorometric competitive immunoassay for chlorpyrifos using rhodamine-modified gold nanoparticles as a label.
Article in Mikrochimica acta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed, 27 citations in OpenAlex.
- The Simultaneous Determination of Chlorpyrifos-Ethyl and -Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay.Biosensors · 2022Article
- A Simple Aptamer SERS Sensor Based on Mesoporous Silica for the Detection of Chlorpyrifos.Foods (Basel, Switzerland) · 2022Article
- A competitive immunoassay for electrochemical impedimetric determination of chlorpyrifos using a nanogold-modified glassy carbon electrode based on enzymatic biocatalytic precipitation.Mikrochimica acta · 2020Article
- Signal amplification in immunoassays by using noble metal nanoparticles: a review.Mikrochimica acta · 2019Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A new kind of labelled antibody was synthesized by modifying gold nanoparticles (AuNPs) with the fluorophore rhodamine B isothiocyanate (RBITC) and a secondary antibody (IgG). The conjugate thus obtained was used in a competitive sandwich assay with a turn-on signal change. It was designed to detect the organophosphorus pesticide chlorpyrifos. The fluorescence of the RBITC-labeled gold immunoprobe with emission at 575 nm and excitation at 556 nm is almost completely quenched. If, however, cysteamine is added, the fluorophore is released from the labeled secondary antibody and fluorescence increases in accordance with the quantity of secondary antibody bound to the sandwich. This assay was applied to determine chlorpyrifos in dried tangerine peels. The detection results were also independently confirmed by LC-MS/MS. The method allows the concentrations of chlorpyrifos to be quantified down to 4.9 ng·mL
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Registered trials
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