Evidence map›Paper›PMID 29594450›Full record

ArticleMikrochimica acta2018

Determination of the activity of alkaline phosphatase by using nanoclusters composed of flower-like cobalt oxyhydroxide and copper nanoclusters as fluorescent probes.

Hai-Bo Wang, Yang Li, Ying Chen, Zi-Ping Zhang, Tian Gan, Yan-Ming Liu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 82 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Hai-Bo WangCollege of Chemistry and Chemical Engineering, Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the Sourth of Henan, Xinyang Normal University, Xinyang, 464000, People's Republic of China. wanghaibohn@163.com.ORCID 0000-0002-8064-1400
Yang LiCollege of Chemistry and Chemical Engineering, Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the Sourth of Henan, Xinyang Normal University, Xinyang, 464000, People's Republic of China.
Ying ChenCollege of Chemistry and Chemical Engineering, Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the Sourth of Henan, Xinyang Normal University, Xinyang, 464000, People's Republic of China.
Zi-Ping ZhangCollege of Life Science, Yantai University, Yantai, 264005, People's Republic of China.
Tian GanInstitute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People's Republic of China. gantianxynu@163.com.
Yan-Ming LiuCollege of Chemistry and Chemical Engineering, Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the Sourth of Henan, Xinyang Normal University, Xinyang, 464000, People's Republic of China.
Xinyang Normal University · CNYantai University · CN

Funding

National Natural Science Foundation of China No. U1704153, 21305119Plan for Scientific Innovation Talent of Henan Province No. 2017JR0016Plan for Young Excellent Teachers in Universities of Henan Province No. 2017GGJSthe Science & Technology Innovation Talents in Universities of Henan Province No. 16HASTIT004
6 · The paper itself

Abstract

The authors describe a sensitive fluorometric method for the determination of the activity of alkaline phosphatase (ALP). It is based on the use of a composite prepared consisting of flower-like cobalt oxyhydroxide (CoOOH) and copper nanoclusters (CuNCs). On formation of the CuNC-CoOOH aggregates, the fluorescence of the CuNCs is quenched by the CoOOH sheets. If, however, the CoOOH sheets are reduced to Co(II) ions in the presence of ascorbic acid (AA), fluorescence recovers. AA is formed in-situ by hydrolysis of the substrate ascorbic acid 2-phosphate (AA2P) as catalyzed by ALP. Thus, the ALP activity can be detected indirectly by kinetic monitoring of the increase in fluorescence, best at excitation/emission wavelengths of 335/410 nm. The assay allows ALP to be determined in 0.5 to 150 mU·mL

Indexed as

Alkaline PhosphataseAscorbic AcidCobaltCopperFluorescent DyesFluorometryHumansOxidesAlkaline PhosphataseAscorbic AcidCobaltCopperFluorescent DyesHCoO2OxidesAlkaline phosphatase activity detectionAscorbic acid 2-phosphateBiosensorCobalt(II)CoOOH sheetsCuNC-CoOOH aggregatesFluorescence recoveryFluorometric assay

Identifiers

PMID29594450
OpenAlexW2783190262

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.