ArticleMikrochimica acta2017
Fluorometric aptamer assay for ochratoxin A based on the use of single walled carbon nanohorns and exonuclease III-aided amplification.
Article in Mikrochimica acta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Aptamers used for biosensors and targeted therapy.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2020Pooled it
- A Label-Free Aptasensor for Turn-On Fluorescent Detection of Aflatoxin B1 Based on an Aggregation-Induced-Emission-Active Probe and Single-Walled Carbon Nanohorns.Foods (Basel, Switzerland) · 2023Article
- A smartphone-assisted colorimetric aptasensor based on aptamer and gold nanoparticles for visual, fast and sensitive detection of ZEN in maize.Food chemistry: X · 2023Article
- Analysis of aptamer-target binding and molecular mechanisms by thermofluorimetric analysis and molecular dynamics simulation.Frontiers in chemistry · 2023Article
- Recent trends in analysis of mycotoxins in food using carbon-based nanomaterials.Journal of food and drug analysis · 2022Review
- Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins.Nanomaterials (Basel, Switzerland) · 2021Review
- Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJToxins · 2020Article
- Article
- A fluorometric aptamer-based assay for ochratoxin A by using exonuclease III-assisted recycling amplification.Mikrochimica acta · 2019Article
- A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials.Mikrochimica acta · 2019Review
- Colorimetric zearalenone assay based on the use of an aptamer and of gold nanoparticles with peroxidase-like activity.Mikrochimica acta · 2018Article
- A test strip for ochratoxin A based on the use of aptamer-modified fluorescence upconversion nanoparticles.Mikrochimica acta · 2018Article
- A fluorometric aptamer-based assay for ochratoxin A using magnetic separation and a cationic conjugated fluorescent polymer.Mikrochimica acta · 2018Article
- Fluorometric aptamer-based determination of ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.Mikrochimica acta · 2018Article
- Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC.Mikrochimica acta · 2018Article
- Exonuclease-assisted multicolor aptasensor for visual detection of ochratoxin A based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorod.Mikrochimica acta · 2018Article
- Fluorometric aptamer based assay for ochratoxin A based on the use of exonuclease III.Mikrochimica acta · 2018Article
- A multifunctional probe based on the use of labeled aptamer and magnetic nanoparticles for fluorometric determination of adenosine 5'-triphosphate.Mikrochimica acta · 2018Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The authors describe an aptamer based assay for the food mycotoxin ochratoxin A (OTA). It is based on the use of exonuclease III (Exo III) which assists in signal amplification, and of single-walled carbon nanohorns (SWCNHs) which act as quenchers of fluorescence. The detection scheme employs a hairpin probe (HP) and a signal probe (SP) labeled with carboxyfluorescein (FAM) at its 5'-end. The fluorescence of intact SPs (best measured at excitation/emission wavelengths of 495/518 nm) is quenched by SWCNHs. The HP contains the OTA-specific aptamer sequence and is partially complementary to the SP. After addition of OTA, the aptamer binds OTA and thus exposes a single-stranded sequence that can hybridize with the SP. Exo III digests the SP to liberate the free fluorophore labels. The damaged SPs no longer are adsorbed by the SWCNHs so that fluorescence is no longer quenched. The method has a detection range that is linear from 10 nM to 1000 nM (with a correlation coefficient of 0.997). The limit of detection (LOD), calculated on the basis of a signal to noise ratio of 3, is 4.2 nM. The procedure was validated by the quantitation of OTA in spiked real samples and were found to be free of interference by the sample matrix. Recoveries ranged from 93.8 to 113.0% in beer and from 92.0 to115.9% in red wine. Graphical abstract After adding ochratoxin A (OTA), the aptamer region in hairpin probe (HP) combined with OTA and thus exposed a single-stranded sequence to hybridize with signal probe (SP). Exonuclease III (Exo III) digested SP to liberate the free fluorophore (FAM).
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