ArticleBio-protocol2018
Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia.
Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Circuit-constrained molecular neuroethology in the medicinal leech Hirudo verbana.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- Kilohertz volumetric imaging of in vivo dynamics using squeezed light field microscopy.Nature methods · 2025Article
- Current Practice in Using Voltage Imaging to Record Fast Neuronal Activity: Successful Examples from Invertebrate to Mammalian Studies.Biosensors · 2023Review
- Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits.eLife · 2021Article
- Electrophysiology and transcriptomics reveal two photoreceptor classes and complex visual integration inThe Journal of experimental biology · 2019Article
- Non-synaptic Plasticity in Leech Touch Cells.Frontiers in physiology · 2019Article
- A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech.eLife · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In this protocol, we introduce an effective method for voltage-sensitive dye (VSD) loading and imaging of leech ganglia as used in Tomina and Wagenaar (2017). Dissection and dye loading procedures are the most critical steps toward successful whole-ganglion VSD imaging. The former entails the removal of the sheath that covers neurons in the segmental ganglion of the leech, which is required for successful dye loading. The latter entails gently flowing a new generation VSD, VF2.1(OMe).H, onto both sides of the ganglion simultaneously using a pair of peristaltic pumps. We expect the described techniques to translate broadly to wide-field VSD imaging in other thin and relatively transparent nervous systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.