ArticleBio-protocol2017
Biochemical Isolation of Myonuclei from Mouse Skeletal Muscle Tissue.
Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
5 citing papers in PubMed.
- Alterations of the skeletal muscle nuclear proteome after acute exercise reveals a posttranscriptional influence.American journal of physiology. Cell physiology · 2025Article
- Article
- Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion.iScience · 2021Article
- Satellite Cell Depletion Disrupts Transcriptional Coordination and Muscle Adaptation to Exercise.Function (Oxford, England) · 2021Article
- Proteomic analysis reveals that wildtype and alanine-expanded nuclear poly(A)-binding protein exhibit differential interactions in skeletal muscle.The Journal of biological chemistry · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Skeletal muscle provides the contractile force necessary for movement, swallowing, and breathing and, consequently, is necessary for survival. Skeletal muscle cells are unique in that they are extremely large cells containing thousands of nuclei. These nuclei must all work in concert to maintain skeletal muscle function and thereby maintain life. The nucleus is a major site of signaling integration and gene expression regulation. However, examining nuclear processes in skeletal muscle can be difficult because myonuclei are challenging to isolate. We optimized a protocol to purify myonuclei from whole muscle tissue using ultracentrifugation over a discontinuous sucrose gradient to separate the nuclear fraction. We used these purified nuclei for downstream applications including flow cytometry and mass spectrometry. We used this method to compare the myonuclear proteome of young and old mouse hindlimb muscles (Cutler
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Registered trials
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