Evidence map›Paper›PMID 29593778›Full record

ArticleFrontiers in genetics2018

Evaluation of Reference Genes to Analyze Gene Expression in Silverside

Tony L R Silveira, William B Domingues, Mariana H Remião, Lucas Santos, Bruna Barreto, Ingrid M Lessa, Antonio Sergio Varela Junior, Diego Martins Pires, Carine Corcini, Tiago Collares and 3 more

Abstract read
In one paragraph

Article in Frontiers in genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Tony L R SilveiraLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
William B DominguesLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Mariana H RemiãoLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Lucas SantosLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Bruna BarretoLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Ingrid M LessaLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Antonio Sergio Varela JuniorInstitute of Biological Sciences, Federal University of Rio Grande, Rio Grande, Brazil.
Diego Martins PiresVeterinary Faculty, Federal University of Pelotas, Pelotas, Brazil.
Carine CorciniVeterinary Faculty, Federal University of Pelotas, Pelotas, Brazil.
Tiago CollaresLaboratory of Cancer Biotechnology, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Fabiana K SeixasLaboratory of Cancer Biotechnology, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.
Ricardo B RobaldoLaboratory of Physiology, Institute of Biology, Federal University of Pelotas, Pelotas, Brazil.
Vinicius F CamposLaboratory of Structural Genomics, Biotechnology Graduate Program, Federal University of Pelotas, Pelotas, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Some mammalian reference genes, which are widely used to normalize the qRT-PCR, could not be used for this purpose due to its high expression variation. The normalization with false reference genes leads to misinterpretation of results. The silversides (

Indexed as

algorithmexpressionfishgenenormalizationreal time PCRsequencingvalidation

Identifiers

PMID29593778
PMCPMC5861154

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.