ArticleEmerging microbes & infections2018
Three Hcp homologs with divergent extended loop regions exhibit different functions in avian pathogenic Escherichia coli.
Article in Emerging microbes & infections, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- TagP, a PAAR-domain containing protein, plays roles in the fitness and virulence ofFrontiers in cellular and infection microbiology · 2024Article
- Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.Microbiology (Reading, England) · 2023Review
- Paradoxical Activation of a Type VI Secretion System Phospholipase Effector by Its Cognate Immunity Protein.Journal of bacteriology · 2023Article
- Article
- Hcp2a of APEC affects mRNA splicing and protein quality control in DF-1 cells.BMC veterinary research · 2022Article
- Hcp Proteins of the Type VI Secretion System Promote Avian PathogenicLife (Basel, Switzerland) · 2022Article
- Molecular Investigation of Outer Membrane Channel Genes Among Multidrug Resistance ClinicalReports of biochemistry & molecular biology · 2022Article
- Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenicFrontiers in microbiology · 2022Review
- Fur functions as an activator of T6SS-mediated bacterial dominance and virulence inFrontiers in microbiology · 2022Article
- VasH Contributes to Virulence ofFrontiers in veterinary science · 2021Article
- Article
- The Ferric Uptake Regulator Represses Type VI Secretion System Function by Binding Directly to theInfection and immunity · 2019Article
- The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli.Veterinary research · 2019Article
- Type VI Secretion System in PathogenicFrontiers in microbiology · 2019Review
- Confirmed and Potential Roles of Bacterial T6SSs in the Intestinal Ecosystem.Frontiers in microbiology · 2019Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type VI secretion systems (T6SSs) contribute to the pathogenicity of avian pathogenic Escherichia coli (APEC), one of the leading causative agents of sepsis and meningitis in poultry. The Hcp protein is a core component of the T6SS tail tube and acts as an exported receptor and a chaperone of effectors. In this study, four distinct Hcp types (Ia, Ib, IIa, and IIb) were designated in Gram-negative bacteria, three of which were widely distributed in APEC. We detected divergence in transcription levels among three hcp clusters in 50% duck serum and demonstrated that hcp1 was upregulated by relieving Fur repression. Further analyses revealed that the host serum could activate the hcp2B operon by H-NS derepression to transcribe the downstream xmtU/xmtV pair for inter-bacterial antagonism. Notably, in a structural analysis based on the genetic classification, Hcp proteins exhibited significant differences in the extended loop regions, suggesting that these regions were related to their functional properties. Indeed, the variant region Vs2 (Loop L2, 3) in Hcp1 and Hcp2B was essential for the delivery of antibacterial effectors and the inhibition of macrophage phagocytosis. Further analyses using a duck model indicated that these Hcps play different roles in the pathogenic processes of APEC and immunoprotection. These results indicated that the functional differentiation of Hcp homologs was driven by differences in transcriptional regulation, extended loop regions, and effector delivery.
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