ArticlePLoS pathogens2018
Molecularly barcoded Zika virus libraries to probe in vivo evolutionary dynamics.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
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- Zika virus diversity in mice is maintained during early vertical transmission from placenta to fetus, but reduced in fetal bodies and brains at late stages of infection.PLoS neglected tropical diseases · 2023Article
- Wolbachia-mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressures.PLoS neglected tropical diseases · 2023Article
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- Gain without pain: Adaptation and increased virulence of Zika virus in vertebrate host without fitness cost in mosquito vector.bioRxiv : the preprint server for biology · 2023Article
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- Interpreting and de-noising genetically engineered barcodes in a DNA virus.PLoS computational biology · 2022Article
- Zika virus persistence in the male macaque reproductive tract.PLoS neglected tropical diseases · 2022Article
- Reinventing positive-strand RNA virus reverse genetics.Advances in virus research · 2022Article
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- Use of hemagglutinin and neuraminidase amplicon-based high-throughput sequencing with variant analysis to detect co-infection and resolve identical consensus sequences of seasonal influenza in a university setting.BMC infectious diseases · 2021Article
- African-Lineage Zika Virus Replication Dynamics and Maternal-Fetal Interface Infection in Pregnant Rhesus Macaques.Journal of virology · 2021Article
- Previous exposure to dengue virus is associated with increased Zika virus burden at the maternal-fetal interface in rhesus macaques.PLoS neglected tropical diseases · 2021Article
- Rapid evolution of enhanced Zika virus virulence during direct vertebrate transmission chains.Journal of virology · 2021Article
- Population bottlenecks and founder effects: implications for mosquito-borne arboviral emergence.Nature reviews. Microbiology · 2021Review
Corrections and comments
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Authors and funding
29 authors at 3 institutions in 2 countries.
Funding
Abstract
Defining the complex dynamics of Zika virus (ZIKV) infection in pregnancy and during transmission between vertebrate hosts and mosquito vectors is critical for a thorough understanding of viral transmission, pathogenesis, immune evasion, and potential reservoir establishment. Within-host viral diversity in ZIKV infection is low, which makes it difficult to evaluate infection dynamics. To overcome this biological hurdle, we constructed a molecularly barcoded ZIKV. This virus stock consists of a "synthetic swarm" whose members are genetically identical except for a run of eight consecutive degenerate codons, which creates approximately 64,000 theoretical nucleotide combinations that all encode the same amino acids. Deep sequencing this region of the ZIKV genome enables counting of individual barcodes to quantify the number and relative proportions of viral lineages present within a host. Here we used these molecularly barcoded ZIKV variants to study the dynamics of ZIKV infection in pregnant and non-pregnant macaques as well as during mosquito infection/transmission. The barcoded virus had no discernible fitness defects in vivo, and the proportions of individual barcoded virus templates remained stable throughout the duration of acute plasma viremia. ZIKV RNA also was detected in maternal plasma from a pregnant animal infected with barcoded virus for 67 days. The complexity of the virus population declined precipitously 8 days following infection of the dam, consistent with the timing of typical resolution of ZIKV in non-pregnant macaques and remained low for the subsequent duration of viremia. Our approach showed that synthetic swarm viruses can be used to probe the composition of ZIKV populations over time in vivo to understand vertical transmission, persistent reservoirs, bottlenecks, and evolutionary dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.