ArticleCell & bioscience2018
Enhanced calcium ion mobilization in osteoblasts on amino group containing plasma polymer nanolayer.
Article in Cell & bioscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
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- The Association of Nanostructured Carbonated Hydroxyapatite with Denatured Albumin and Platelet-Rich Fibrin: Impacts on Growth Factors Release and Osteoblast Behavior.Journal of functional biomaterials · 2024Article
- Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.Journal of biological engineering · 2023Article
- Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density.Molecules (Basel, Switzerland) · 2023Article
- An Evaluation of Parylene Thin Films to Prevent Encrustation for a Urinary Bladder Pressure MEMS Sensor System.Polymers · 2023Article
- Optimized Gingiva Cell Behavior on Dental Zirconia as a Result of Atmospheric Argon Plasma Activation.Materials (Basel, Switzerland) · 2023Article
- Laser Structured Dental Zirconium for Soft Tissue Cell Occupation-Importance of Wettability Modulation.Materials (Basel, Switzerland) · 2022Article
- Review
- The nanomorphology of cell surfaces of adhered osteoblasts.Beilstein journal of nanotechnology · 2021Article
- Efficacy of Plasma-Polymerized Allylamine Coating of Zirconia after Five Years.Journal of clinical medicine · 2020Article
- Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges.Frontiers in bioengineering and biotechnology · 2020Article
- Surface phosphonation treatment shows dose-dependent enhancement of the bioactivity of polyetheretherketone.RSC advances · 2019Article
- Plasma Polymerized Allylamine-The Unique Cell-Attractive Nanolayer for Dental Implant Materials.Polymers · 2019Article
- In Vitro Comparative Study of Oxygen Plasma Treated Poly(Lactic⁻Co⁻Glycolic) (PLGA) Membranes and Supported Nanostructured Oxides for Guided Bone Regeneration Processes.Materials (Basel, Switzerland) · 2018Article
- Calcium signaling: an underlying link between cardiac disease and carcinogenesis.Cell & bioscience · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiomaterial modifications-chemical and topographical-are of particular importance for the integration of materials in biosystems. Cells are known to sense these biomaterial characteristics, but it has remained unclear which physiological processes bio modifications trigger. Hence, the question arises of whether the dynamic of intracellular calcium ions is important for the characterization of the cell-material interaction. In our prior research we could demonstrate that a defined geometrical surface topography affects the cell physiology; this was finally detectable in a reduced intracellular calcium mobilization after the addition of adenosine triphosphate (ATP).
resultsThis new contribution examines the cell physiology of human osteoblasts concerning the relative cell viability and the calcium ion dynamic on different chemical modifications of silicon-titanium (Ti) substrates. Chemical modifications comprising the coating of Ti surfaces with a plasma polymerized allylamine (PPAAm)-layer or with a thin layer of collagen type-I were compared with a bare Ti substrate as well as tissue culture plastic. For this purpose, the human osteoblasts (MG-63 and primary osteoblasts) were seeded onto the surfaces for 24 h. The relative cell viability was determined by colorimetric measurements of the cell metabolism and relativized to the density of cells quantified using crystal violet staining. The calcium ion dynamic of osteoblasts was evaluated by the calcium imaging analysis of fluo-3 stained vital cells using a confocal laser scanning microscope. The positively charged nano PPAAm-layer resulted in enhanced intracellular calcium ion mobilization after ATP-stimulus and cell viability. This study underlines the importance of the calcium signaling for the manifestation of the cell physiology.
conclusionsOur current work provides new insights into the intracellular calcium dynamic caused by diverse chemical surface compositions. The calcium ion dynamic appears to be a sensitive parameter for the cell physiology and, thus, may represent a useful approach for evaluating a new biomaterial. In this regard, reliable in vitro-tests of cell behavior at the interface to a material are crucial steps in securing the success of a new biomaterial in medicine.
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