ArticleBiotechnology for biofuels2018
Enhancing the catalytic activity of a novel GH5 cellulase
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 86 citations in OpenAlex.
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- CRISPR/Cas9 mediated targeted knock-in of eglA gene to improve endoglucanase activity of Aspergillus fumigatus LMB-35Aa.Scientific reports · 2024Article
- Review
- Dynamics of loops surrounding the active site architecture in GH5_2 subfamily TfCel5A for cellulose degradation.Biotechnology for biofuels and bioproducts · 2023Article
- Modification of the 4-Hydroxyphenylacetate-3-hydroxylase Substrate Pocket to Increase Activity towards Resveratrol.Molecules (Basel, Switzerland) · 2023Article
- Production, purification, characterization and application of two novel endoglucanases from buffalo rumen metagenome.Journal of animal science and biotechnology · 2023Article
- Characterization of aspartokinase double mutants using a combination of experiments and simulations.Heliyon · 2023Article
- Engineering cellulases for conversion of lignocellulosic biomass.Protein engineering, design & selection : PEDS · 2023Review
- Dissecting the essential role of N-glycosylation in catalytic performance of xanthan lyase.Bioresources and bioprocessing · 2022Article
- Immobilization of Penicillin G Acylase on Vinyl Sulfone-Agarose: An Unexpected Effect of the Ionic Strength on the Performance of the Immobilization Process.Molecules (Basel, Switzerland) · 2022Article
- Identification and Mutation Analysis of Nonconserved Residues on the TIM-Barrel Surface of GH5_5 Cellulases for Catalytic Efficiency and Stability Improvement.Applied and environmental microbiology · 2022Article
- Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01.Scientific reports · 2022Article
- Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability.Bioprocess and biosystems engineering · 2022Article
- Fungal cellulases: protein engineering and post-translational modifications.Applied microbiology and biotechnology · 2022Review
- Screening, cloning, enzymatic properties of a novel thermostable cellulase enzyme, and its potential application on water hyacinth utilization.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Current Status of Mining, Modification, and Application of Cellulases in Bioactive Substance Extraction.Current issues in molecular biology · 2021Review
- A multi-functional genetic manipulation system and its use in high-level expression of a β-mannanase mutant with high specific activity in Pichia pastoris.Microbial biotechnology · 2021Article
- Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution.Biotechnology for biofuels · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCellulases of glycosyl hydrolase (GH) family 5 share a (β/α)
resultsIn the present study, an acidic, mesophilic GH5 cellulase (with optimal activity at pH 4.0 and 70 °C),
conclusionThis study proposed a novel cellulase with potentials for industrial application. A specific position was identified to play key roles in cellulase-substrate interactions and enzyme catalysis. It is of great importance for understanding the binding mechanism of GH5 cellulases, and provides an effective strategy to improve the catalytic performance of cellulases.
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Registered trials
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