ArticleBMC complementary and alternative medicine2018
Species identification approach for both raw materials and end products of herbal supplements from Tinospora species.
Article in BMC complementary and alternative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances.Chinese medicine · 2022Review
- Article
- Article
- Using high-resolution melting to identify Calliphoridae (blowflies) species from Brazil.PeerJ · 2020Article
- The DNA-Based Authentication of Commercial Herbal Products Reveals Their Globally Widespread Adulteration.Frontiers in pharmacology · 2019Review
- Article
- Multiuse of Bar-HRM for Ophiocordyceps sinensis identification and authentication.Scientific reports · 2018Article
- Bar-HRM for authenticating soursop (Annona muricata) tea.Scientific reports · 2018Article
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Authors and funding
3 authors.
Funding
Abstract
backgroundNowadays herbal products used in traditional medicine are sold in processed forms and thus morphological authentication is almost impossible. With herbal industry rapidly growing size, consumer safety becomes an important issue that requires special attention. Identification of herbal species in the products is therefore needed.
methodsSequences from the selected regions (matK, rbcL, trnL and ITS1) were retrieved and analysed. Then the most suitable barcode was assessed for discrimination of T. crispa from closely related species by HRM analysis and used in authentication of commercial products.
resultsThe ITS1 barcode was found to be the suitable primer as melting data from the HRM assay proved to be capable of distinguishing T. crispa from its related species. The developed protocol was then employed to authenticate medicinal products in powdered form. HRM analysis of all tested samples here revealed that five out of eight products contained not only the indicated species T. crispa but also other Tinospora, that have a high level of morphological similarity.
conclusionMisrepresentation, poor packaging and inappropriate labeling of the tested medicinal herbal products are thought to be the reason of the results here. Using Bar-HRM with the ITS marker lead to success in authenticating the tested herbal products.
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