Observational studyVirology journal2018
HPV genotype determination and E6/E7 mRNA detection for management of HPV positive women.
Observational study in Virology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Small extracellular vesicle-mediated transfer of HPV16 transcripts from Ca Ski cells increases proliferation of HPV-negative recipient cancer cells.Cell communication and signaling : CCS · 2026Article
- HPV persistence and CIN2 + Detection in women with normal cytology and Non-16/18 High-Risk HPV positivity.BMC women's health · 2026Article
- Human papilloma virus (HPV) mediated cancers: an insightful update.Journal of translational medicine · 2025Review
- Article
- Post-conization pathological upgrading and outcomes of 466 patients with low-grade cervical intraepithelial neoplasia.Frontiers in oncology · 2024Article
- Diagnostic value of high-risk HPV E6/E7 mRNA in patients with ASCUS.BMC women's health · 2023Article
- Article
- Oral HPV Infection in Women with HPV-Positive Cervix Is Closely Related to Oral Sex.Diagnostics (Basel, Switzerland) · 2023Article
- Performance of p16/Ki67 Immunostaining for Triage of Elderly Women with Atypical Squamous Cells of Undetermined Significance.Journal of clinical medicine · 2023Article
- E6/E7 mRNA Expression of the Most Prevalent High-Risk HPV Genotypes in Cervical Samples from Serbian Women.Diagnostics (Basel, Switzerland) · 2023Article
- Spontaneous regression of cervical intraepithelial neoplasia 3 in women with a biopsy-cone interval of greater than 11 weeks.BMC cancer · 2022Article
- Article
- Possible role of negative human papillomavirus E6/E7 mRNA as a predictor of regression of cervical intraepithelial neoplasia 2 lesions in hr-HPV positive women.Virology journal · 2022Article
- Adherence to the American Society for Colposcopy and Cervical Pathology guidelines: an observational study.Obstetrics & gynecology science · 2022Article
- Evidence for HPV DNA in the placenta of women who resorted to elective abortion.BMC pregnancy and childbirth · 2021Article
- Immunohistochemical Detection of Human Papillomavirus 16 E7 Oncoprotein in Cervical Lesions.Journal of Cancer · 2021Article
- Progression of CIN1/LSIL HPV Persistent of the Cervix: Actual Progression or CIN3 Coexistence.Infectious diseases in obstetrics and gynecology · 2021Article
- Conservative management of CIN2 p16 positive lesions in women with multiple HPV infection.BMC infectious diseases · 2020Article
- Multiple HPV 16 infection with two strains: a possible marker of neoplastic progression.BMC cancer · 2020Article
- HPV16 persistent infection and recurrent disease after LEEP.Virology journal · 2019Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClinical management of HPV positive women is difficult since many of the infections, including high-risk oncogene genotypes (hr-HPV), are transient. Therefore only a limited number of patients have a high-grade lesion and sending all HPV positive women for colposcopy would only increase costs and unnecessary treatment, with serious psychological consequences for patients. The need has emerged to identify other HPV related markers able to correctly detect women with a high-risk of developing high-grade lesions. Genotyping and the search for E6/E7 mRNA are among the possible candidates.
methodsThe study was carried out by means of an observational analysis of the data relative to 674 HR-HPV positive women who we had observed from January 2013 to June 2015; the data had been gathered in a database at the HPV Center of the University Hospital of Catania, Italy. Women were considered eligible for this study if the following data was present in the database: Pap TEST, histologic evaluation, HPV TEST and E6/E7 mRNA detection. We calculated the Odds Ratio (OR) of woman who were mRNA positive, with CIN2+ lesions, and Odds Ratio of HPV16 positive women.
resultsTranscripts were detected in 23.6% (69/292) of the women with CIN1 and in 97.2% (210/220) of those with CIN2 + . Regarding genotyping, the 81,8% (180/220) of the women with CIN2+ had genotype 16, while only 18.1% (40/220) had genotype 18, 31, 33, 45. We calculated the OR in the group of HPV16 women with CIN2+ (OR = 4.62; 95% CI = 3.13 to 6.82), this value increased (OR = 106.12; 95% CI = 53.71 to 209.69) in women with CIN2+ and positive mRNA. DISCUSSION: The presence of the HPV16 genotype in our study was associated with a risk 5 times greater of developing a high-grade lesion (CIN2+) (OR = 4.62 95% CI:3.13-6.82); this supports the hypothesis that it would be opportune to have targeted protocols for the management of HPV 16 positive women. The results showed that there was an association between E6/E7 mRNA expression and histology (OR = 106.12; 95% CI = 53.71 to 209.69). The E6/E7 mRNA test showed a higher prevalence of E6 and E7 transcripts in patients with higher-grade lesions.
conclusionThe results of this study suggest that the HPV genotype determination and E6/E7 mRNA detection would find an important application for management of HPV positive women.
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