ArticleBMC plant biology2018
Identification of cold stress responsive microRNAs in two winter turnip rape (Brassica rapa L.) by high throughput sequencing.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Identification of Freezing-Responsive microRNAs and Their Targets in Chinese Jujube by Small RNA and Degradome Sequencing.International journal of molecular sciences · 2026Article
- Integrated physiological and multi-omics analyses reveal genotype-specific GABA responses associated with salt tolerance inFrontiers in plant science · 2026Article
- Rice and shine: a review of miRNA-mediated responses to abiotic stress inFrontiers in plant science · 2026Review
- Optimization of Chromosome Preparation and Karyotype Analysis of Winter Turnip Rape (International journal of molecular sciences · 2025Article
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- Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Changes Underlying Adaptation to Low Temperature ofPlants (Basel, Switzerland) · 2024Article
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- MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon (Plants (Basel, Switzerland) · 2024Article
- microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms.International journal of molecular sciences · 2024Review
- Identifying genetic variation associated with environmental gradients and drought-tolerance phenotypes in ponderosa pine.Ecology and evolution · 2023Article
- Small RNA and Degradome Sequencing in Floral Bud Reveal Roles of miRNAs in Dormancy Release ofInternational journal of molecular sciences · 2023Article
- Glutathione imparts stress tolerance againstPlant signaling & behavior · 2022Article
- Application of RNAi technology: a novel approach to navigate abiotic stresses.Molecular biology reports · 2022Review
- Identification of microRNAs That Provide a Low Light Stress Tolerance-Mediated Signaling Pathway during Vegetative Growth in Rice.Plants (Basel, Switzerland) · 2022Article
- MicroRNA398: A Master Regulator of Plant Development and Stress Responses.International journal of molecular sciences · 2022Review
- Review
- Integrated sRNA-seq and RNA-seq Analyses Reveal a microRNA Regulation Network Involved in Cold Response inGenes · 2022Article
- Article
- Genetic Mechanisms of Cold Signaling in Wheat (Life (Basel, Switzerland) · 2022Review
- A Chromosome Level Genome Assembly of a Winter Turnip Rape (Frontiers in plant science · 2022Article
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8 authors.
Funding
Abstract
backgroundLow temperature is a major abiotic stress affecting the production of rapeseed in China by impeding plant growth and development. A comprehensive knowledge of small-RNA expression pattern in Brassica rapa under cold stress could improve our knowledge of microRNA-mediated stress responses.
resultsA total of 353 cold-responsive miRNAs, 84 putative novel and 269 conserved miRNAs, were identified from the leaves and roots of two winter turnip rape varieties 'Longyou 7' (cold-tolerant) and 'Tianyou 4' (cold-sensitive), which were stressed under - 4 °C for 8 h. Eight conserved (miR166h-3p-1, miR398b-3p, miR398b-3p-1, miR408d, miR156a-5p, miR396h, miR845a-1, miR166u) and two novel miRNAs (Bra-novel-miR3153-5p and Bra-novel-miR3172-5p) were differentially expressed in leaves of 'Longyou 7' under cold stress. Bra-novel-miR3936-5p was up-regulated in roots of 'Longyou 7' under cold stress. Four and five conserved miRNAs were differentially expressed in leaves and roots of 'Tianyou 4' after cold stress. Besides, we found two conserved miRNAs (miR319e and miR166m-2) were down-regulated in non-stressed roots of 'Longyou 7' compared with 'Tianyou 4'. After cold stress, we found two and eight miRNAs were differentially expressed in leaves and roots of 'Longyou 7' compared with 'Tianyou 4'. The differentially expressed miRNAs between two cultivars under cold stress include novel miRNAs and the members of the miR166 and miR319 families. A total of 211 target genes for 15 known miRNAs and two novel miRNAs were predicted by bioinformatic analysis, mainly involved in metabolic processes and stress responses. Five differentially expressed miRNAs and predicted target genes were confirmed by quantitative reverse transcription PCR, and the expressional changes of target genes were negatively correlated to differentially expressed miRNAs. Our data indicated that some candidate miRNAs (e.g., miR166e, miR319, and Bra-novel-miR3936-5p) may play important roles in plant response to cold stress.
conclusionsOur work indicates that miRNA and putative target genes mediated metabolic processes and stress responses are significant to cold tolerance in B. rapa.
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