ArticlePLoS genetics2018
Experimental evolution of diverse Escherichia coli metabolic mutants identifies genetic loci for convergent adaptation of growth rate.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Article
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- Mutations Elevate an Underground Pathway to a Physiologically Relevant Protopathway.Molecular biology and evolution · 2025Article
- Modulating bacterial function utilizing A knowledge base of transcriptional regulatory modules.Nucleic acids research · 2024Article
- The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions.mSystems · 2024Article
- Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and tradeoffs.Nature communications · 2023Article
- The hallmarks of a tradeoff in transcriptomes that balances stress and growth functions.Research square · 2023Article
- Adaptation to novel spatially-structured environments is driven by the capsule and alters virulence-associated traits.Nature communications · 2022Article
- An automated DIY framework for experimental evolution of Pseudomonas putida.Microbial biotechnology · 2021Article
- Changes in the distribution of fitness effects and adaptive mutational spectra following a single first step towards adaptation.Nature communications · 2021Article
- Transcriptional regulator-induced phenotype screen reveals drug potentiators in Mycobacterium tuberculosis.Nature microbiology · 2021Article
- Extreme Antagonism Arising from Gene-Environment Interactions.Biophysical journal · 2020Article
- Metabolic role of pyrophosphate-linked phosphofructokinase pfk for C1 assimilation in Methylotuvimicrobium alcaliphilum 20Z.Microbial cell factories · 2020Article
- Evolutionary Repair Experiments as a Window to the Molecular Diversity of Life.Current biology : CB · 2020Review
- Growth of E. coli on formate and methanol via the reductive glycine pathway.Nature chemical biology · 2020Article
- Effects of ordered mutations on dynamics in signaling networks.BMC medical genomics · 2020Article
- Adaptive laboratory evolution ofMicrobiology (Reading, England) · 2020Article
- Short-Term Adaptation Modulates Anaerobic Metabolic Flux to Succinate by Activating ExuT, a Novel D-Glucose Transporter inFrontiers in microbiology · 2020Article
- Hidden resources in theProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Deciphering the essentiality and function of the anti-σMolecular microbiology · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Cell growth is determined by substrate availability and the cell's metabolic capacity to assimilate substrates into building blocks. Metabolic genes that determine growth rate may interact synergistically or antagonistically, and can accelerate or slow growth, depending on genetic background and environmental conditions. We evolved a diverse set of Escherichia coli single-gene deletion mutants with a spectrum of growth rates and identified mutations that generally increase growth rate. Despite the metabolic differences between parent strains, mutations that enhanced growth largely mapped to core transcription machinery, including the β and β' subunits of RNA polymerase (RNAP) and the transcription elongation factor, NusA. The structural segments of RNAP that determine enhanced growth have been previously implicated in antibiotic resistance and in the control of transcription elongation and pausing. We further developed a computational framework to characterize how the transcriptional changes that occur upon acquisition of these mutations affect growth rate across strains. Our experimental and computational results provide evidence for cases in which RNAP mutations shift the competitive balance between active transcription and gene silencing. This study demonstrates that mutations in specific regions of RNAP are a convergent adaptive solution that can enhance the growth rate of cells from distinct metabolic states.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.