ArticleInternational journal of molecular sciences2018
Co-Culture with Human Osteoblasts and Exposure to Extremely Low Frequency Pulsed Electromagnetic Fields Improve Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of Low-Frequency Electromagnetic Fields on Mesenchymal Stem Cells Differentiation: A Systematic Review.Tissue engineering and regenerative medicine · 2022Pooled it
- Review
- Fabrication and characterization of an anti-corrosion/infection VAN@PLGA/HA/CMCS composite coating on magnesium alloy.Journal of materials science. Materials in medicine · 2025Article
- The effects and mechanisms of electromagnetic fields on bone remodeling: From clinical to laboratory.Journal of orthopaedic translation · 2025Review
- Insights into bone and cartilage responses to pulsed electromagnetic field stimulation: a review with quantitative comparisons.Frontiers in bioengineering and biotechnology · 2025Review
- Establishment of a human 3D in vitro liver-bone model as a potential system for drug toxicity screening.Archives of toxicology · 2025Article
- Augmentation of Deficient Bone Healing by Pulsed Electromagnetic Fields-From Mechanisms to Clinical Outcomes.Bioengineering (Basel, Switzerland) · 2024Review
- Pulsating electromagnetic fields for perineal lacerations and surgical wounds healing in the postpartum: a pilot study.Archives of gynecology and obstetrics · 2024Article
- Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024Review
- Deciphering the Cell-Specific Effect of Osteoblast-Macrophage Crosstalk in Periodontitis.Tissue engineering. Part A · 2023Article
- Article
- Electromagnetic field exposure as a plausible approach to enhance the proliferation and differentiation of mesenchymal stem cells in clinically relevant scenarios.Journal of Zhejiang University. Science. B · 2022Review
- Mineralized collagen scaffold bone graft accelerate the osteogenic process of HASCs in proper concentration.Regenerative therapy · 2021Article
- Direct Current Electrical Fields Improve Experimental Wound Healing by Activation of Cytokine Secretion and Erk1/2 Pathway Stimulation.Life (Basel, Switzerland) · 2021Article
- Modulation of Macrophage Activity by Pulsed Electromagnetic Fields in the Context of Fracture Healing.Bioengineering (Basel, Switzerland) · 2021Article
- Anti-Oxidative and Immune Regulatory Responses of THP-1 and PBMC to Pulsed EMF Are Field-Strength Dependent.International journal of environmental research and public health · 2021Article
- Effect of Muscle Cell Preservation on Viability and Differentiation of Hamstring Tendon Graft In Vitro.Cells · 2021Article
- Pulsed Electromagnetic Field Stimulation of Bone Healing and Joint Preservation: Cellular Mechanisms of Skeletal Response.Journal of the American Academy of Orthopaedic Surgeons. Global research & reviews · 2020Review
- Primary Human Chondrocytes Affected by Cigarette Smoke-Therapeutic Challenges.International journal of molecular sciences · 2020Article
- Asperosaponin VI stimulates osteogenic differentiation of rat adipose-derived stem cells.Regenerative therapy · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human adipose-derived mesenchymal stem cells (Ad-MSCs) have been proposed as suitable option for cell-based therapies to support bone regeneration. In the bone environment, Ad-MSCs will receive stimuli from resident cells that may favor their osteogenic differentiation. There is recent evidence that this process can be further improved by extremely low frequency pulsed electromagnetic fields (ELF-PEMFs). Thus, the project aimed at (i) investigating whether co-culture conditions of human osteoblasts (OBs) and Ad-MSCs have an impact on their proliferation and osteogenic differentiation; (ii) whether this effect can be further improved by repetitive exposure to two specific ELF-PEMFs (16 and 26 Hz); (iii) and the effect of these ELF-PEMFs on human osteoclasts (OCs). Osteogenic differentiation was improved by co-culturing OBs and Ad-MSCs when compared to the individual mono-cultures. An OB to Ad-MSC ratio of 3:1 had best effects on total protein content, alkaline phosphatase (AP) activity, and matrix mineralization. Osteogenic differentiation was further improved by both ELF-PEMFs investigated. Interestingly, only repetitive exposure to 26 Hz ELF-PEMF increased Trap5B activity in OCs. Considering this result, a treatment with gradually increasing frequency might be of interest, as the lower frequency (16 Hz) could enhance bone formation, while the higher frequency (26 Hz) could enhance bone remodeling.
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