Evidence map›Paper›PMID 29581727›Full record

ArticlePlant methods2018

Reference gene identification for reliable normalisation of quantitative RT-PCR data in

Duc Quan Nguyen, Andrew L Eamens, Christopher P L Grof

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.

  1. Pooled it
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  5. Article
  6. Molecular Characterization of Resistance to Nicosulfuron inInternational journal of molecular sciences · 2023
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  9. Multi-omics intervention inFrontiers in plant science · 2022
    Review
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  12. Genes · 2020
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Duc Quan NguyenCentre for Plant Science, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308 Australia.
Andrew L Eamens *Centre for Plant Science, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308 Australia.
Christopher P L Grof *Centre for Plant Science, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308 Australia.
University of Newcastle Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundQuantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entirely dependent on the identification of reliable reference genes for data normalisation. Green foxtail (

resultsEleven putative candidate reference genes were identified and examined across thirteen different

conclusionsThis approach readily demonstrated the suitably of the three selected reference genes for the accurate and reliable normalisation of

Indexed as

C4 cropGene expressionQuantitative RT-PCR normalisationReference genesSetaria viridis

Identifiers

PMID29581727
PMCPMC5861610
OpenAlexW2791691203

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.