ArticleMicrobial cell factories2018
Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 103 citations in OpenAlex.
- Genetic engineering of cyanophages for harmful cyanobacterial bloom treatment-A mini review.Synthetic and systems biotechnology · 2027Review
- Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.mSystems · 2026Article
- Design and Implementation of a Blue-Light-Controlled Gene-Switch System.Molecules (Basel, Switzerland) · 2026Article
- Cyanobacterial Biofertilizer Production by Guanidine-Producing Enzymes.ACS synthetic biology · 2026Review
- Mangrove cyanobacterial diversity as a source of bioactive natural products.Current research in microbial sciences · 2026Article
- Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.bioRxiv : the preprint server for biology · 2025Article
- CyanoStat: An open-source platform for COHardwareX · 2025Article
- Greening the Production of Indigo Blue Exploiting Light and a Recombinant Synechocystis sp. PCC6803 Strain Expressing the Enzyme mFMO.Microbial biotechnology · 2025Article
- Investigation of themBio · 2025Article
- Co-expression of auxiliary genes enhances the activity of a heterologous OBiotechnology for biofuels and bioproducts · 2025Article
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review.Environmental science and ecotechnology · 2024Review
- Role of natural transformation in the evolution of small cryptic plasmids in Synechocystis sp. PCC 6803.Environmental microbiology reports · 2023Article
- Design of synthetic promoters for cyanobacteria with generative deep-learning model.Nucleic acids research · 2023Article
- The current situations and limitations of genetic engineering in cyanobacteria: a mini review.Molecular biology reports · 2023Review
- Identification of a Far-Red Light-Inducible Promoter that Exhibits Light Intensity Dependency and Reversibility in a Cyanobacterium.ACS synthetic biology · 2023Article
- Light and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials.Materials today. Bio · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- Life in biophotovoltaics systems.Frontiers in plant science · 2023Review
- Engineering plant family TPS into cyanobacterial host for terpenoids production.Plant cell reports · 2022Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO
resultsWe systematically characterized endogenous genetic elements of Synechocystis 6803, including promoters, ribosome binding sites, transcription terminators, and plasmids. Expression from twelve native promoters was compared by measuring fluorescence from the reporter protein EYFP in an identical setup, exhibiting an 8000-fold range of promoter activities. Moreover, we measured the strength of twenty native ribosome binding sites and eight native terminators, indicating their influence on the expression of the reporter genes. In addition, two shuttle vectors, pCA-UC118 and pCB-SC101, capable of replication in both Synechocystis 6803 and E. coli were constructed. Expression of reporter proteins were significantly enhanced in cells containing these new plasmids, thus providing superior gene expression platforms in this cyanobacterium.
conclusionsThe results of this study provide useful and well characterized native tools for bioengineering work in the model cyanobacterium Synechocystis 6803.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.