ArticlePloS one2018
Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 51 citations in OpenAlex.
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- Selection and validation of reference genes for quantitative real-time PCR during the developmental stages of seeds inFrontiers in plant science · 2025Article
- Cross-species stability of reference genes in medicinal plants Arnica montana and Arnica chamissonis.Scientific reports · 2024Article
- Development and evaluation of suitable reference genes for qRT-PCR normalization of hybrids derived from Lycium barbarum and Lycium ruthenicum.Molecular biology reports · 2024Article
- Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Identification of Reliable Reference Genes under Different Stresses and in Different Tissues ofGenes · 2022Article
- Identification and Expression Analysis of NAC Gene Family in Weeping Trait ofPlants (Basel, Switzerland) · 2022Article
- Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata.Scientific reports · 2022Article
- Selection and validation of reference genes for RT-qPCR analysis of different organs at various development stages inOpen life sciences · 2022Article
- Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata.Scientific reports · 2021Article
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- Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions.Scientific reports · 2021Article
- Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica.Molecular biology reports · 2021Article
- Screening of optimal reference genes for qRT-PCR and preliminary exploration of cold resistance mechanisms in Prunus mume and Prunus sibirica varieties.Molecular biology reports · 2020Article
- Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plants under differential biotic stresses.Scientific reports · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time polymerase chain reaction (qRT-PCR) is a prevalent method for gene expression analysis, depending on the stability of the reference genes for data normalization. Lagerstroemia indica and L. speciosa are popular ornamental plants which are famous for the long flowering period. However, no systematic studies on reference genes in Lagerstroemia have yet been conducted. In the present study, we selected nine candidate reference genes (GAPDH, TUA, TUB, 18S, RPII, EF-1α, ATC, EIF5A and CYP) and evaluated their expression stability in different tissues during floral development of L. indica and L. speciosa using four algorithms (geNorm, NormFinder, BestKeeper and, RefFinder). Results showed that RPII and EF-1α were the most stably expressed and suitable reference genes for both of Lagerstroemia species. Moreover, ACT exhibited high expression stability in L. indica and GAPDH was a suitable reference gene for L. speciosa in different flower development stages. TUB was an unsuitable reference gene for gene expression normalization due to significant variations in expression across all samples. Finally, we verified the reliability of the selected candidate reference genes by amplifying an AGAMOUS homolog (LsAG1) of Arabidopsis thaliana. This study provides a list of suitable reference genes, thereby broadening the genetic basis of the gene expression patterns in Lagerstroemia species.
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