ArticleAnalytical chemistry2018
Microfluidic Chip with Integrated Electrophoretic Immunoassay for Investigating Cell-Cell Interactions.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets.Sensors (Basel, Switzerland) · 2026Article
- Online affinity micro free-flow electrophoresis for the continuous monitoring of insulinLab on a chip · 2026Article
- Artificial Intelligence-Aided Microfluidic Cell Culture Systems.Biosensors · 2025Review
- Identifying and minimizing primary sources of temporal broadening in online affinity micro free-flow electrophoresis.The Analyst · 2025Article
- Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon.Electrophoresis · 2024Article
- Lab-on-a-chip: an advanced technology for the modernization of traditional Chinese medicine.Chinese medicine · 2024Review
- Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit.Biosensors & bioelectronics · 2024Article
- Evaluation of Surface Treatments of PDMS Microfluidic Devices for Improving Small-Molecule Recovery with Application to Monitoring Metabolites Secreted from Islets of Langerhans.ACS measurement science au · 2023Article
- Insulin detection in diabetes mellitus: challenges and new prospects.Nature reviews. Endocrinology · 2023Review
- Islet-on-a-chip for the study of pancreatic β-cell function.In vitro models · 2022Review
- Smartphone-microfluidic fluorescence imaging system for studying islet physiology.Frontiers in endocrinology · 2022Article
- The Foundation for Engineering a Pancreatic Islet Niche.Frontiers in endocrinology · 2022Review
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Making quantitative biomicrofluidics from microbore tubing and 3D-printed adapters.Biomicrofluidics · 2021Article
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- Towards an Insulin Resistant Adipose Model on a Chip.Cellular and molecular bioengineering · 2021Article
- A Smartphone-Fluidic Digital Imaging Analysis System for Pancreatic Islet Mass Quantification.Frontiers in bioengineering and biotechnology · 2021Article
- A Parallel Perifusion Slide From Glass for the Functional and Morphological Analysis of Pancreatic Islets.Frontiers in bioengineering and biotechnology · 2021Article
- Challenging Bioanalyses with Capillary Electrophoresis.Analytical chemistry · 2020Review
- Synchronized stimulation and continuous insulin sensing in a microfluidic human Islet on a Chip designed for scalable manufacturing.Lab on a chip · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microfluidics have been used to create "body-on-chip" systems to mimic in vivo cellular interactions with a high level of control. Most such systems rely on optical observation of cells as a readout. In this work we integrated a cell-cell interaction chip with online microchip electrophoresis immunoassay to monitor the effects of the interaction on protein secretion dynamics. The system was used to investigate the effects of adipocytes on insulin secretion. Chips were loaded with 190 000 3T3-L1 adipocytes and a single islet of Langerhans in separate chambers. The chambers were perfused at 300-600 nL/min so that adipocyte secretions flowed over the islets for 3 h. Adipocytes produced 80 μM of nonesterified fatty acids (NEFAs), a factor known to impact insulin secretion, at the islets. After perfusion, islets were challenged with a step change in glucose from 3 to 11 mM while monitoring insulin secretion at 8 s intervals by online immunoassay. Adipocyte treatment augmented insulin secretion by 6-fold compared to controls. The effect was far greater than comparable concentrations of NEFA applied to the islets demonstrating that adipocytes release multiple factors that can strongly potentiate insulin secretion. The experiments reveal that integration of chemical analysis with cell-cell interaction can provide valuable insights into cellular functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.