ArticleNature methods2018
Burden-driven feedback control of gene expression.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 191 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
191 citing papers in PubMed.
- Engineering a genetic construct to express soluble proteins tethered to the cell membrane.Bioscience reports · 2026Article
- Programmable promoter editing for precise control of transgene expression.Nature biotechnology · 2026Article
- Regulation of Escherichia coli fermentation processes: from molecular mechanisms to smart industrial practice.Archives of microbiology · 2026Review
- Regulating microbial cell factories to resist ROS stress in chemical production.Archives of microbiology · 2026Review
- Burden-aware feedback control of microbial consortia.Nature communications · 2026Article
- Off-gas capture: a promising strategy for removal and recovery of toxic bioproducts in aerobic fermentation.Biotechnology for biofuels and bioproducts · 2026Article
- Overriding Bioprocess Perturbations With a Cell-Machine Interface for Reliable Microbial Stress-Response Control.Microbial biotechnology · 2026Article
- Dissecting host stress responses for predictable heterologous gene expression in E. coli.Nucleic acids research · 2026Article
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- Exploring the computing power of microbes that shapes the environment.Current opinion in microbiology · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Integration-coupled activation of promoterless combinatorial pathway libraries inSynthetic biology (Oxford, England) · 2026Article
- Going beyond size: exploring the metabolic burden inSynthetic biology (Oxford, England) · 2026Article
- Tuning evolvability via plasmid copy number and regulatory architecture.Nature communications · 2025Article
- Article
- Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability.Nature communications · 2025Article
- Repurposing the bacterial surface display technology for drug delivery.Advanced drug delivery reviews · 2025Review
- Disentangling Metabolite Detection from Pathway Stress Using Biosensors.ACS synthetic biology · 2025Article
- Genetic controllers for enhancing the evolutionary longevity of synthetic gene circuits in bacteria.Nature communications · 2025Article
- CRISPRi-Linked Multimodule Negative Feedback Loops to Address Winner-Take-All Resource Competition.ACS synthetic biology · 2025Article
131 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Cells use feedback regulation to ensure robust growth despite fluctuating demands for resources and differing environmental conditions. However, the expression of foreign proteins from engineered constructs is an unnatural burden that cells are not adapted for. Here we combined RNA-seq with an in vivo assay to identify the major transcriptional changes that occur in Escherichia coli when inducible synthetic constructs are expressed. We observed that native promoters related to the heat-shock response activated expression rapidly in response to synthetic expression, regardless of the construct. Using these promoters, we built a dCas9-based feedback-regulation system that automatically adjusts the expression of a synthetic construct in response to burden. Cells equipped with this general-use controller maintained their capacity for native gene expression to ensure robust growth and thus outperformed unregulated cells in terms of protein yield in batch production. This engineered feedback is to our knowledge the first example of a universal, burden-based biomolecular control system and is modular, tunable and portable.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.