ArticleJournal of biomedical materials research. Part A2018
Effects of antigen removal on a porcine osteochondral xenograft for articular cartilage repair.
Article in Journal of biomedical materials research. Part A, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- A Systematic Review and Guide to Mechanical Testing for Articular Cartilage Tissue Engineering.Tissue engineering. Part C, Methods · 2019Pooled it
- Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices.Bioengineering (Basel, Switzerland) · 2025Review
- From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage.Bioengineering (Basel, Switzerland) · 2025Article
- Applications in osteochondral organoids for osteoarthritis research: from pathomimetic modeling to tissue engineering repair.Frontiers in bioengineering and biotechnology · 2025Review
- Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts.Cell and tissue banking · 2024Article
- High-Speed Centrifugation Efficiently Removes Immunogenic Elements in Osteochondral Allografts.Orthopaedic surgery · 2024Article
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Cell Therapy Approaches for Articular Cartilage Regeneration.Organogenesis · 2023Review
- Article
- Osteochondral Tissue Engineering: The Potential of Electrospinning and Additive Manufacturing.Pharmaceutics · 2021Review
- Graphene Oxide-Doped Gellan Gum-PEGDA Bilayered Hydrogel Mimicking the Mechanical and Lubrication Properties of Articular Cartilage.Advanced healthcare materials · 2021Article
- Reducing porcine corneal graft rejection, with an emphasis on porcine endogenous retrovirus transmission safety: a review.International journal of ophthalmology · 2019Review
- Comparison of various reagents for preparing a decellularized porcine cartilage scaffold.American journal of translational research · 2019Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Given the limited availability of fresh osteochondral allografts and uncertainty regarding performance of decellularized allografts, this study was undertaken as part of an effort to develop an osteochondral xenograft for articular cartilage repair. The purpose was to evaluate a simple antigen removal procedure based mainly on treatment with SDS and nucleases. Histology demonstrated a preservation of collagenous structure and removal of most nuclei. Immunohistochemistry revealed the apparent retention of α-Gal within osteocyte lacunae unless the tissue underwent an additional α-galactosidase processing step. Cytoplasmic protein was completely removed as shown by Western blot. Quantitatively, the antigen removal protocol was found to extract approximately 90% of DNA from cartilage and bone, and it extracted over 80% of glycosaminoglycan from cartilage. Collagen content was not affected. Mechanical testing of cartilage and bone were performed separately, in addition to testing the cartilage-bone interface, and the main effect of antigen removal was an increase in cartilage hydraulic permeability. In vivo immunogenicity was assessed by subcutaneous implantation into DBA/1 J mice, and the response was typical of a foreign body rather than immune reaction. Thus, an osteochondral xenograft produced as described has the potential for further development into a treatment for osteochondral lesions in the human knee. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2251-2260, 2018.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.