ArticlePlant biotechnology journal2018
Efficient genome editing of wild strawberry genes, vector development and validation.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A spatiotemporal single-cell transcriptomic atlas reveals fertilization-induced auxin signaling driving strawberry fruit set and development.Nature communications · 2026Article
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- Rapid screening of genome edited strawberry (Fragaria ×ananassa) regenerants using high-resolution melting analysis followed by Amplicon sequencing.BMC research notes · 2026Article
- Whitening fruit by CRISPR/Cas9-mediated homoeolog-specific gene editing ofHorticulture research · 2026Article
- Recent advances in biosynthesis and regulation of strawberry anthocyanins.Horticulture research · 2025Article
- The aldehyde (ALD) locus controls C6-aldehyde production in kiwifruit and affects consumer perception of fruit aroma.Plant physiology · 2025Article
- Agroinfiltration technique for elucidating the functions of strawberry genes in Fragaria vesca.Scientific reports · 2025Article
- A native visual screening reporter-assisted CRISPR/Cas9 system for high-efficient genome editing in strawberry.Molecular horticulture · 2025Article
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Improving gene editing of CRISPR/Cas9 using the callus-specific promoterFrontiers in plant science · 2025Article
- Changes in soluble sugars and the expression of sugar transporter protein genes in strawberry crowns responding toPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- High-temperature stress in strawberry: understanding physiological, biochemical and molecular responses.Planta · 2024Review
- The importance of genotyping within the climate-smart plant breeding value chain - integrative tools for genetic enhancement programs.Frontiers in plant science · 2024Review
- Advances in genomics and genome editing for improving strawberry (Frontiers in genetics · 2024Review
- Improvement of crop production in controlled environment agriculture through breeding.Frontiers in plant science · 2024Review
- Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry.Plant methods · 2023Article
- Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale.International journal of molecular sciences · 2023Article
- Article
- CRISPR/Cas9 editing of the polygalacturonaseHorticulture research · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system is an effective genome editing tool for plant and animal genomes. However, there are still few reports on the successful application of CRISPR-Cas9 to horticultural plants, especially with regard to germ-line transmission of targeted mutations. Here, we report high-efficiency genome editing in the wild strawberry Fragaria vesca and its successful application to mutate the auxin biosynthesis gene TAA1 and auxin response factor 8 (ARF8). In our CRISPR system, the Arabidopsis U6 promoter AtU6-26 and the wild strawberry U6 promoter FveU6-2 were each used to drive the expression of sgRNA, and both promoters were shown to lead to high-efficiency genome editing in strawberry. To test germ-line transmission of the edited mutations and new mutations induced in the next generation, the progeny of the primary (T0) transgenic plants carrying the CRISPR construct was analysed. New mutations were detected in the progeny plants at a high efficiency, including large deletions between the two PAM sites. Further, T1 plants harbouring arf8 homozygous knockout mutations grew considerably faster than wild-type plants. The results indicate that our CRISPR vectors can be used to edit the wild strawberry genome at a high efficiency and that both sgRNA design and appropriate U6 promoters contribute to the success of genomic editing. Our results open up exciting opportunities for engineering strawberry and related horticultural crops to improve traits of economic importance.
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