ArticlePeerJ2018
Assessing soil bacterial community and dynamics by integrated high-throughput absolute abundance quantification.
Article in PeerJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Functional and Mechanistic Analysis of Wild Badger-Derived Lactiplantibacillus Plantarum Y40 Against Salmonella Infection.Probiotics and antimicrobial proteins · 2026Article
- Microbial diversity and water quality changes in mangrove sediments in Quanzhou Bay.Frontiers in microbiology · 2026Article
- Absolute quantitative metagenomic analysis reveals unique gut bacteria underlying berberine and metformin's anti-metabolic disorders effects.Microbiology spectrum · 2025Article
- Artificial Root Exudates Affect Soil Phosphorus Activation in an Acidic Soil: Role of Phosphorus-Solubilizing Bacteria via RNA Sequencing.Current microbiology · 2025Article
- Year-Round Quantification, Structure and Dynamics of Epibacterial Communities From Diverse Macroalgae Reveal a Persistent Core Microbiota and Strong Host Specificities.Environmental microbiology reports · 2025Article
- Assessment of absolute abundance in mother-infant gut microbiome using marine-sourced bacterial DNA spike-in and comparison with conventional quantification methods.Microbiome research reports · 2025Article
- Synthetic DNA spike-in standards for cross-domain absolute quantification of microbiomes by rRNA gene amplicon sequencing.ISME communications · 2025Article
- The relict plantFrontiers in plant science · 2025Article
- Synthetic spike-in metabarcoding for plant pathogen diagnostics results in precise quantification of copy number within the genusISME communications · 2025Article
- Revealing novel insights into the enhancement of quality in black tea processing through microbial intervention.Food chemistry: X · 2024Article
- The Fecal Redox Potential in Healthy and Diarrheal Pigs and Their Correlation with Microbiota.Antioxidants (Basel, Switzerland) · 2024Article
- Enrichment of different taxa of the enigmatic candidate phyla radiation bacteria using a novel picolitre droplet technique.ISME communications · 2024Article
- The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor-Null Mice.Nutrients · 2023Article
- Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China.Microorganisms · 2023Article
- Antibiotic resistance genes and bacterial community distribution patterns in pig farms.Folia microbiologica · 2022Article
- Article
- Current Applications of Absolute Bacterial Quantification in Microbiome Studies and Decision-Making Regarding Different Biological Questions.Microorganisms · 2021Review
- Integrating Systems and Synthetic Biology to Understand and Engineer Microbiomes.Annual review of biomedical engineering · 2021Review
- Relative Abundances of Species or Sequence Variants Can Be Misleading: Soil Fungal Communities as an Example.Microorganisms · 2021Article
- Relative and Quantitative Rhizosphere Microbiome Profiling Results in Distinct Abundance Patterns.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial ecological studies have been remarkably promoted by the high-throughput sequencing approach with explosive information of taxonomy and relative abundance. However, relative abundance does not reflect the quantity of the microbial community and the inter-sample differences among taxa. In this study, we refined and applied an integrated high-throughput absolute abundance quantification (iHAAQ) method to better characterize soil quantitative bacterial community through combining the relative abundance (by high-throughput sequencing) and total bacterial quantities (by quantitative PCR). The proposed iHAAQ method was validated by an internal reference strain EDL933 and a laboratory strain WG5. Application of the iHAAQ method to a soil phenanthrene biodegradation study showed that for some bacterial taxa, the changes of relative and absolute abundances were coincident, while for others the changes were opposite. With the addition of a microbial activity inhibitor (NaN
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.