ArticleLight, science & applications2017
Two-color multiphoton
Article in Light, science & applications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.Biomedical optics express · 2024Article
- Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window.bioRxiv : the preprint server for biology · 2024Article
- Pulse train gating to improve signal generation forNeurophotonics · 2023Article
- Pulse train gating to improve signal generation forbioRxiv : the preprint server for biology · 2023Article
- Diamond Raman laser and Yb fiber amplifier forBiomedical optics express · 2022Article
- Evaluation of resonant scanning as a high-speed imaging technique for two-photon imaging of cortical vasculature.Biomedical optics express · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Segmentation-Less, Automated, Vascular Vectorization.PLoS computational biology · 2021Article
- Mathematical synthesis of the cortical circulation for the whole mouse brain-part II: Microcirculatory closure.Microcirculation (New York, N.Y. : 1994) · 2021Article
- Hyperspectral multiphoton microscopy forOptica · 2020Article
- Effect of vascular structure on laser speckle contrast imaging.Biomedical optics express · 2020Article
- Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020Review
- Article
- Efficient non-degenerate two-photon excitation for fluorescence microscopy.Optics express · 2019Article
- Measurement of the relative non-degenerate two-photon absorption cross-section for fluorescence microscopy.Optics express · 2019Article
- Polymer dots enable deepBiomedical optics express · 2019Article
- Dual-color deep-tissue three-photon microscopy with a multiband infrared laser.Light, science & applications · 2018Article
- Skull optical clearing window forLight, science & applications · 2018Article
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Authors and funding
10 authors.
Funding
Abstract
Two-color multiphoton microscopy through wavelength mixing of synchronized lasers has been shown to increase the spectral window of excitable fluorophores without the need for wavelength tuning. However, most currently available dual output laser sources rely on the costly and complicated optical parametric generation approach. In this report, we detail a relatively simple and low cost diamond Raman laser pumped by a ytterbium fiber amplifier emitting at 1055 nm, which generates a first Stokes emission centered at 1240 nm with a pulse width of 100 fs. The two excitation wavelengths of 1055 and 1240 nm, along with the effective two-color excitation wavelength of 1140 nm, provide an almost complete coverage of fluorophores excitable within the range of 1000-1300 nm. When compared with 1055 nm excitation, two-color excitation at 1140 nm offers a 90% increase in signal for many far-red emitting fluorescent proteins (for example, tdKatushka2). We demonstrate multicolor imaging of tdKa-tushka2 and Hoechst 33342 via simultaneous two-color two-photon, and two-color three-photon microscopy in engineered 3D multicellular spheroids. We further discuss potential benefits and applications for two-color three-photon excitation. In addition, we show that this laser system is capable of
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.