ArticleInternational journal of mass spectrometry2018
Subnanogram proteomics: impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples.
Article in International journal of mass spectrometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Northern peatland microbial communities exhibit resistance to warming and acquire electron acceptors from soil organic matter.Nature communications · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Digital Microfluidics for Sample Preparation in Low-Input Proteomics.Small methods · 2025Review
- Multicolumn Nanoflow Liquid Chromatography with Accelerated Offline Gradient Generation for Robust and Sensitive Single-Cell Proteome Profiling.Analytical chemistry · 2024Article
- What's new in single-cell proteomics.Current opinion in biotechnology · 2024Review
- A critical evaluation of ultrasensitive single-cell proteomics strategies.Analytical and bioanalytical chemistry · 2024Review
- Separation methods in single-cell proteomics: RPLC or CE?International journal of mass spectrometry · 2022Article
- Three-dimensional feature matching improves coverage for single-cell proteomics based on ion mobility filtering.Cell systems · 2022Article
- A Sensitive and Controlled Data-Independent Acquisition Method for Proteomic Analysis of Cell Therapies.Journal of proteome research · 2022Article
- Application of nanomaterials in proteomics-driven precision medicine.Theranostics · 2022Review
- IceR improves proteome coverage and data completeness in global and single-cell proteomics.Nature communications · 2021Article
- Ultrasensitive NanoLC-MS of Subnanogram Protein Samples Using Second Generation Micropillar Array LC Technology with Orbitrap Exploris 480 and FAIMS PRO.Analytical chemistry · 2021Article
- Simple and Efficient Microsolid-Phase Extraction Tip-Based Sample Preparation Workflow to Enable Sensitive Proteomic Profiling of Limited Samples (200 to 10,000 Cells).Journal of proteome research · 2021Article
- Deep Profiling of Microgram-Scale Proteome by Tandem Mass Tag Mass Spectrometry.Journal of proteome research · 2021Article
- Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell.Chemical science · 2020Article
- Improved Sensitivity of Ultralow Flow LC-MS-Based Proteomic Profiling of Limited Samples Using Monolithic Capillary Columns and FAIMS Technology.Analytical chemistry · 2020Article
- Single-cell Proteomics: Progress and Prospects.Molecular & cellular proteomics : MCP · 2020Review
- Picoflow Liquid Chromatography-Mass Spectrometry for Ultrasensitive Bottom-Up Proteomics Using 2-μm-i.d. Open Tubular Columns.Analytical chemistry · 2020Article
- Mass Spectrometry Advances and Perspectives for the Characterization of Emerging Adoptive Cell Therapies.Molecules (Basel, Switzerland) · 2020Review
- Improved Single-Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
One of the greatest challenges for mass spectrometry (MS)-based proteomics is the limited ability to analyze small samples. Here we investigate the relative contributions of liquid chromatography (LC), MS instrumentation and data analysis methods with the aim of improving proteome coverage for sample sizes ranging from 0.5 ng to 50 ng. We show that the LC separations utilizing 30-μm-
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.