ArticleNeuron2018
Role for Wnt Signaling in Retinal Neuropil Development: Analysis via RNA-Seq and In Vivo Somatic CRISPR Mutagenesis.
Article in Neuron, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 93 citations in OpenAlex.
- Protocadherin 9 Promotes Cell Survival of Different Bipolar Cell Types in the Developing Mouse Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Ankyrin-B and ankyrin-G are required for synaptic integrity in the mouse outer retina.PNAS nexus · 2026Article
- Cerebral Hypoperfusion Causes Behavioral Changes and Impairs the Rod Photoreceptor Pathway in the Retina of Aged Mice.Cellular and molecular neurobiology · 2026Article
- Spatiotemporal dynamics of ecto-5'-nucleotidase (CD73) in mouse retina under physiological conditions.Development (Cambridge, England) · 2026Article
- Utilizing a culture system for horizontal cells to study neural circuit assembly in the developing mouse retina.Frontiers in cellular neuroscience · 2026Article
- Homeostatic scaling of dynorphin signaling by a non-canonical opioid receptor.Nature communications · 2025Article
- Advancements in CRISPR/Cas systems for disease treatment.Acta pharmaceutica Sinica. B · 2025Review
- Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization.Nature communications · 2025Article
- Molecular mechanism establishing the OFF pathway in vision.Nature communications · 2025Article
- Enhanced Transcriptional Activation in Developing Mouse Photoreceptors.Investigative ophthalmology & visual science · 2025Article
- Cardiac Applications of CRISPR/AAV-Mediated Precise Genome Editing.bioRxiv : the preprint server for biology · 2024Article
- Developmental control of rod number via a light-dependent retrograde pathway from intrinsically photosensitive retinal ganglion cells.Developmental cell · 2024Article
- Article
- Synaptotagmin-9 in mouse retina.Visual neuroscience · 2024Article
- Murine Retina Outer Plexiform Layer Development and Transcriptome Analysis of Pre-Synapses in Photoreceptors.Life (Basel, Switzerland) · 2024Article
- Presynaptic Proteins and Their Roles in Visual Processing by the Retina.Annual review of vision science · 2024Review
- Non-canonical Wnt pathway expression in the developing mouse and human retina.Experimental eye research · 2024Article
- Article
- Na,K-ATPase activity promotes macropinocytosis in colon cancer via Wnt signaling.Biology open · 2024Article
- Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina.Cell reports · 2024Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Screens for genes that orchestrate neural circuit formation in mammals have been hindered by practical constraints of germline mutagenesis. To overcome these limitations, we combined RNA-seq with somatic CRISPR mutagenesis to study synapse development in the mouse retina. Here synapses occur between cellular layers, forming two multilayered neuropils. The outer neuropil, the outer plexiform layer (OPL), contains synapses made by rod and cone photoreceptor axons on rod and cone bipolar dendrites, respectively. We used RNA-seq to identify selectively expressed genes encoding cell surface and secreted proteins and CRISPR-Cas9 electroporation with cell-specific promoters to assess their roles in OPL development. Among the genes identified in this way are Wnt5a and Wnt5b. They are produced by rod bipolars and activate a non-canonical signaling pathway in rods to regulate early OPL patterning. The approach we use here can be applied to other parts of the brain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.