Evidence map›Paper›PMID 29576318›Full record

ArticleStructure (London, England : 1993)2018

A Structural Model of the Urease Activation Complex Derived from Ion Mobility-Mass Spectrometry and Integrative Modeling.

Joseph D Eschweiler, Mark A Farrugia, Sugyan M Dixit, Robert P Hausinger, Brandon T Ruotolo

Abstract read
In one paragraph

Article in Structure (London, England : 1993), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. A Flexible, Modular Platform for Multidimensional Ion Mobility of Native-like Ions.Journal of the American Society for Mass Spectrometry · 2023
    Article
  8. Article
  9. Protein Structure Prediction with Mass Spectrometry Data.Annual review of physical chemistry · 2022
    Review
  10. Article
  11. Iron-Containing Ureases.Coordination chemistry reviews · 2021
    Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Sumner's legacy: a century of urease crystals and recent structural advances.Critical reviews in biochemistry and molecular biology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joseph D EschweilerDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Mark A FarrugiaDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
Sugyan M DixitDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Robert P HausingerDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Brandon T RuotoloDepartment of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: bruotolo@umich.edu.

Funding

Next-generation Mass Spectrometry Technologies for Integrated Structural Proteomics- RenewalR01GM095832 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RUOTOLO, BRANDON T · 2011 to 2024
$5.8M
NIGMS NIH HHS R01 GM095832
6 · The paper itself

Abstract

The synthesis of active Klebsiella aerogenes urease via an 18-subunit enzyme apoprotein-accessory protein pre-activation complex has been well studied biochemically, but thus far this complex has remained refractory to direct structural characterization. Using ion mobility-mass spectrometry, we characterized several protein complexes between the core urease apoprotein and its accessory proteins, including the 610-kDa (UreABC)

Indexed as

Amino Acid MotifsBacterial ProteinsBinding SitesCloning, MolecularEnterobacter aerogenesEnzyme ActivationEscherichia coliGene ExpressionGenetic VectorsIon Mobility SpectrometryKineticsModels, MolecularProtein BindingProtein Interaction Domains and MotifsProtein SubunitsRecombinant ProteinsBacterial ProteinsProtein SubunitsRecombinant ProteinsUreasecomputational modelingintegrative structural biologyion mobilitynative mass spectrometryurease assembly

Identifiers

PMID29576318
PMCPMC5884726

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.