ReviewAnalytical and bioanalytical chemistry2018
Critical assessment of digital PCR for the detection and quantification of genetically modified organisms.
Review in Analytical and bioanalytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 135 citations in OpenAlex.
- Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.GM crops & food · 2026Article
- Digital PCR in forensic genetics: principles, applications, and challenges.International journal of legal medicine · 2026Review
- Digital PCR for the Authentication of KAMUTFoods (Basel, Switzerland) · 2026Article
- Digital PCR-based characterization of certified genomic DNA reference materials from genetically modified papaya Huanong No. 1.BMC biotechnology · 2026Article
- Development of a novel duplex crystal digital PCR for the detection of PRRSV-1 and PRRSV-2.Frontiers in cellular and infection microbiology · 2026Article
- A standard-curve-free ΔΔCt method for rapid GMO compliance screening relative to labeling thresholds.Frontiers in plant science · 2026Article
- Multiplex Quantification of 19 GM Soybean Lines Using Digital PCR.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Harmonization trial onThe journal of liquid biopsy · 2025Article
- Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information.Communications biology · 2025Article
- Detection of Soybean GMO Events Using Two Multiplex Droplet Digital PCR Assays.Journal of AOAC International · 2025Article
- Transgenic tomato strategies targeting whitefly eggs from apoplastic or ovary-directed proteins.BMC plant biology · 2024Article
- Development and Application of a Multiplex Reverse Transcription-Droplet Digital PCR Assay for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus in Bivalve Shellfish.Foods (Basel, Switzerland) · 2024Article
- High-Resolution Melting (HRM) analysis of DNA methylation using semiconductor chip-based digital PCR.Genes & genomics · 2024Article
- Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis.Diagnostics (Basel, Switzerland) · 2024Review
- Assessment of Common Factors Associated with Droplet Digital PCR (ddPCR) Quantification ofInternational journal of molecular sciences · 2024Article
- A digital PCR approach to assess the purity of oregano.Heliyon · 2024Article
- The Development of a Series of Genomic DNA Reference Materials with Specific Copy Number Ratios for The Detection of Genetically Modified Maize DBN9936.Foods (Basel, Switzerland) · 2024Article
- Digital PCR for Single-Cell Analysis.Biosensors · 2024Review
- Fast and Accurate Multiplex Identification and Quantification of Seven Genetically Modified Soybean Lines Using Six-Color Digital PCR.Foods (Basel, Switzerland) · 2023Article
- A Molecular Toolbox to Identify and Quantify Grape Varieties: On the Trace of "Glera".Foods (Basel, Switzerland) · 2023Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The number of genetically modified organisms (GMOs) on the market is steadily increasing. Because of regulation of cultivation and trade of GMOs in several countries, there is pressure for their accurate detection and quantification. Today, DNA-based approaches are more popular for this purpose than protein-based methods, and real-time quantitative PCR (qPCR) is still the gold standard in GMO analytics. However, digital PCR (dPCR) offers several advantages over qPCR, making this new technique appealing also for GMO analysis. This critical review focuses on the use of dPCR for the purpose of GMO quantification and addresses parameters which are important for achieving accurate and reliable results, such as the quality and purity of DNA and reaction optimization. Three critical factors are explored and discussed in more depth: correct classification of partitions as positive, correctly determined partition volume, and dilution factor. This review could serve as a guide for all laboratories implementing dPCR. Most of the parameters discussed are applicable to fields other than purely GMO testing. Graphical abstract There are generally three different options for absolute quantification of genetically modified organisms (GMOs) using digital PCR: droplet- or chamber-based and droplets in chambers. All have in common the distribution of reaction mixture into several partitions, which are all subjected to PCR and scored at the end-point as positive or negative. Based on these results GMO content can be calculated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.