ArticlePlant molecular biology2018
miRNAs control HAM1 functions at the single-cell-layer level and are essential for normal embryogenesis in Arabidopsis.
Article in Plant molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism.Frontiers in plant science · 2022Pooled it
- HAM1 negatively regulates drought tolerance in Brassica juncea.BMC plant biology · 2026Article
- TheForestry research · 2026Article
- Functions and Regulation of HAM Family Genes in Meristems During Gametophyte and Sporophyte Generations.Plant, cell & environment · 2025Review
- MicroRNA analysis reveals 2 modules that antagonistically regulate xylem tracheary element development in Arabidopsis.The Plant cell · 2024Article
- Article
- The NGATHA-like GenesInternational journal of molecular sciences · 2022Article
- Function and Regulation of microRNA171 in Plant Stem Cell Homeostasis and Developmental Programing.International journal of molecular sciences · 2022Review
- Meristem Initiation andFrontiers in plant science · 2022Review
- Post-transcriptional regulation of seed dormancy and germination: Current understanding and future directions.Plant communications · 2021Review
- Genomic imprinted genes in reciprocal hybrid endosperm of Brassica napus.BMC plant biology · 2021Article
- Integration of Small RNA and Transcriptome Sequencing Reveal the Roles of miR395 and ATP Sulfurylase in Developing Seeds of Chinese Kale.Frontiers in plant science · 2021Article
- Review
- Characterization of the GRAS gene family reveals their contribution to the high adaptability of wheat.PeerJ · 2021Article
- A signal cascade originated from epidermis defines apical-basal patterning of Arabidopsis shoot apical meristems.Nature communications · 2020Article
- MicroRNA Dynamics and Functions During Arabidopsis Embryogenesis.The Plant cell · 2019Article
- Adapting INTACT to analyse cell-type-specific transcriptomes and nucleocytoplasmic mRNA dynamics in the Arabidopsis embryo.Plant reproduction · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
key messagemiR171a controls HAM1 functions within the protodermal cells of the embryo, and these controls are essential for normal embryogenesis in Arabidopsis. Arabidopsis thaliana miR171a is known to bind to and cleave mRNAs of three HAIRY MERISTEM (HAM) genes that encode members of the GRAS family transcriptional regulators. The molecular functions of the HAM genes are still being elucidated in Arabidopsis. However, detailed expression patterns of miR171a and the effects of the failure of miR171a to suppress HAM genes were unknown till now. Here, we show the detailed expression patterns of miR171a and HAM1 using green fluorescent protein and confocal scanning microscopy. Our observations revealed that miR171a was expressed in the surface cell layer of the embryo and shoot apical meristem, and it controlled HAM1 functions. To determine the impact of the failure of miR171a to suppress of HAM1, we introduced seven synonymous mutations into the miR171a target site of the HAM1 gene (modified HAM1, mHAM1) and generated transgenic plants that had mHAM1 driven by HAM1 native promoter. The mHAM1 transgenic plants showed organogenic defects. These results indicate that the control of HAM1 functions at the single-cell-layer level by miR171a is essential for proper organ formation in Arabidopsis.
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